Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 731959..732734 Replicon chromosome
Accession NZ_CP082805
Organism Klebsiella pneumoniae strain SCAID PHRX1-2021 (13/97)

Toxin (Protein)


Gene name TacT2 Uniprot ID B6ZBI6
Locus tag K8B74_RS03615 Protein ID WP_004889762.1
Coordinates 732249..732734 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag K8B74_RS03610 Protein ID WP_004150912.1
Coordinates 731959..732252 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8B74_RS03590 (727168) 727168..727770 - 603 WP_002916735.1 short chain dehydrogenase -
K8B74_RS03595 (727868) 727868..728779 + 912 WP_014906929.1 LysR family transcriptional regulator -
K8B74_RS03600 (728780) 728780..729928 - 1149 WP_014906930.1 PLP-dependent aspartate aminotransferase family protein -
K8B74_RS03605 (729939) 729939..731315 - 1377 WP_014906931.1 pyridoxal-phosphate dependent enzyme -
K8B74_RS03610 (731959) 731959..732252 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
K8B74_RS03615 (732249) 732249..732734 + 486 WP_004889762.1 GNAT family N-acetyltransferase Toxin
K8B74_RS03620 (733438) 733438..734031 + 594 WP_004188553.1 hypothetical protein -
K8B74_RS03625 (734128) 734128..734344 + 217 Protein_714 transposase -
K8B74_RS03635 (735022) 735022..735735 - 714 WP_002916694.1 DUF554 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 734128..734280 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17585.56 Da        Isoelectric Point: 8.5144

>T215143 WP_004889762.1 NZ_CP082805:732249-732734 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDSMMLMVTLRDLVN
A

Download         Length: 486 bp

>T215143 NZ_CP110513:38212-38364 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGTACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT215143 WP_004150912.1 NZ_CP082805:731959-732252 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT215143 NZ_CP110513:c38157-38100 [Escherichia coli]
GCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A447W563

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References