Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 780715..781490 Replicon chromosome
Accession NZ_CP068689
Organism Klebsiella pneumoniae subsp. pneumoniae strain RJBSI76

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag I5002_RS03900 Protein ID WP_004150910.1
Coordinates 781005..781490 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag I5002_RS03895 Protein ID WP_004150912.1
Coordinates 780715..781008 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I5002_RS03875 775923..776525 - 603 WP_062954968.1 short chain dehydrogenase -
I5002_RS03880 776623..777534 + 912 WP_004188550.1 LysR family transcriptional regulator -
I5002_RS03885 777535..778683 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
I5002_RS03890 778694..780070 - 1377 WP_004217775.1 cystathionine beta-synthase -
I5002_RS03895 780715..781008 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
I5002_RS03900 781005..781490 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
I5002_RS03905 782194..782787 + 594 WP_004188553.1 hypothetical protein -
I5002_RS03910 782884..783100 + 217 Protein_769 transposase -
I5002_RS03915 783706..784578 + 873 WP_004188557.1 ParA family protein -
I5002_RS03920 784578..784961 + 384 WP_004150906.1 hypothetical protein -
I5002_RS03925 784954..786321 + 1368 WP_004150905.1 replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 782884..783036 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T187566 WP_004150910.1 NZ_CP068689:781005-781490 [Klebsiella pneumoniae subsp. pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T187566 NZ_CP089924:c294221-293739 [Lysobacter sp. 5GHs7-4]
ATGCACACCCCGCGCTGGTCGTTCCAGCCGCTCAGCGGCGCCGGCGCGGCGATCTCCGGCGGCCGCCTCAATCGGCCCGG
CATCGACGCCCTGTACCTGGCGCTGGAACCGGAAACCGCGGTCGAGGAGTTCCGCCAGGACGATCCGCTGGTGCGCCCCG
GCACCCTGGTCGCCTACCGCGCCACCATCGCGCGCGTGGCCGACTTCCGCGCCGGCTACGACCCCGCGCACTGGCCGCCG
CTGTGGCAGGAACTGTCCTGCAACTGGAAGCGCATGGCGTTCCTGGACGAGATCGAGCCGCCCAGCTGGGTGATCGCCGA
CGACGCGCGCGCCGCCGGCCTGGCCGCGATCGCGTTCCCGTCCACGCGCCGCGCCGGCGGTTCCAACCTGGTGGTCTATC
CCGACCTGCTGGGCGCCGACGACCGCCTGCTGGTGCACGACCCGCGCATGGACCTGCCGATCGATCCGTCCAGCTGGCGC
TGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT187566 WP_004150912.1 NZ_CP068689:780715-781008 [Klebsiella pneumoniae subsp. pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT187566 NZ_CP089924:c294631-294260 [Lysobacter sp. 5GHs7-4]
GTGTCCGCCGCCGCCTTCGACACCTTCCTGGAAACCCTGCGCGAACCCTCGCGCCCGACGCTGTCGCCGGTGCGTATGGC
CCAGGCCCTGCACATGGGCGTGAGCGACCTGGCCGGCCTGGCGCGCGTGCACCGCAACACGGTCACCGGCAATCCGGAGT
CCTCGCAGCTGCAGGGCGCCTTGCGCGACATCGTCAAAGTGCTGGCCGCGGCCTACCGGGTCAACGACGACCGCGAGCGC
ACGCTGTTCTGGTTCATCAATCATCCGATCGCCGAGTTCGGTTACCAGACCGCCGCCGACCTGGTGCGCGACGGCAAATC
CGAGGCGGTGATCCGCTACCTGGCGACGTTGGAAGGCGGCGCCACCGGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References