Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 2934243..2935018 Replicon chromosome
Accession NZ_CP021696
Organism Klebsiella pneumoniae strain AR_0158

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1W1JAQ8
Locus tag AM404_RS14815 Protein ID WP_009308645.1
Coordinates 2934243..2934728 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag AM404_RS14820 Protein ID WP_004150912.1
Coordinates 2934725..2935018 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM404_RS14790 2931405..2932118 + 714 WP_002916694.1 DUF554 domain-containing protein -
AM404_RS14805 2932633..2932849 - 217 Protein_2844 transposase -
AM404_RS14810 2932946..2933539 - 594 WP_004188553.1 hypothetical protein -
AM404_RS14815 2934243..2934728 - 486 WP_009308645.1 GNAT family N-acetyltransferase Toxin
AM404_RS14820 2934725..2935018 - 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
AM404_RS14825 2935661..2937037 + 1377 WP_004174417.1 pyridoxal-phosphate dependent enzyme -
AM404_RS14830 2937048..2938196 + 1149 WP_004174416.1 PLP-dependent aspartate aminotransferase family protein -
AM404_RS14835 2938197..2939108 - 912 WP_004188550.1 LysR family transcriptional regulator -
AM404_RS14840 2939206..2939808 + 603 WP_009308651.1 short chain dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 2932697..2932849 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17595.60 Da        Isoelectric Point: 8.5144

>T77064 WP_009308645.1 NZ_CP021696:c2934728-2934243 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T77064 NZ_CP021696:c2934728-2934243 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGACCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT77064 WP_004150912.1 NZ_CP021696:c2935018-2934725 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT77064 NZ_CP021696:c2935018-2934725 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1W1JAQ8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References