Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 761693..762468 Replicon chromosome
Accession NZ_CP084859
Organism Klebsiella pneumoniae strain SB1139

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag LGM11_RS03835 Protein ID WP_004150910.1
Coordinates 761983..762468 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag LGM11_RS03830 Protein ID WP_004150912.1
Coordinates 761693..761986 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LGM11_RS03810 (756901) 756901..757503 - 603 WP_002916735.1 short chain dehydrogenase -
LGM11_RS03815 (757601) 757601..758512 + 912 WP_004188550.1 LysR family transcriptional regulator -
LGM11_RS03820 (758513) 758513..759661 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
LGM11_RS03825 (759672) 759672..761048 - 1377 WP_004217775.1 cystathionine beta-synthase -
LGM11_RS03830 (761693) 761693..761986 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
LGM11_RS03835 (761983) 761983..762468 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
LGM11_RS03840 (763172) 763172..763766 + 595 Protein_756 hypothetical protein -
LGM11_RS03845 (763863) 763863..764079 + 217 Protein_757 transposase -
LGM11_RS03850 (764686) 764686..765558 + 873 WP_004188557.1 ParA family protein -
LGM11_RS03855 (765558) 765558..765941 + 384 WP_004150906.1 hypothetical protein -
LGM11_RS03860 (765934) 765934..767301 + 1368 WP_004150905.1 SPI-7-type island replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 763863..764015 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T219595 WP_004150910.1 NZ_CP084859:761983-762468 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T219595 NZ_CP116554:c50276-49935 [Enterococcus faecalis]
ATGAGTGACGAAAAGAAATATATTCCTAAAAAGGGCGATATTGTATGGATAGATTTCGATCCTTCAGCTGAAAAAGAAAT
ACAGAAAAGACGTCCAGGTCTAGTAGTTTCTAGATATGAGTTTAATAGAAAGACATTGTTTGCAGTAATTTGTCCCATAA
CTTCTACAATTAAAAATATGCCAACTCGTTATACATTACCTGATGAAATGGAAACTCATGGACAAGTCGTTATTTCCCAA
TTAAAATCTTTAGACTTTGCAGAAAGAAAGTTAAGCCAAATAGAACATTTACCATTAAAGGATATGGCTAAAATAGATCA
AATCATTGAATACATTTTTTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT219595 WP_004150912.1 NZ_CP084859:761693-761986 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT219595 NZ_CP116554:c50506-50276 [Enterococcus faecalis]
ATGTTAACCACAAAATCTAGAAAACAAGGAAGTTCTGTAGTATTGACATTACCATCTAATAACGGACAGAAACCAAAAGC
AGACCAAGAATATATAGTGATGTATTCTGATGATGGGACAATTACGTTAGTCCCTAAATTACAAGACCCATTTAGTGGTG
GATCAGAAGGGGAGTACTACGAAAAAGATGAATGGCATGACATTGAACCTGGAGGGAGAGAATTATTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References