Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 2582265..2583040 Replicon chromosome
Accession NZ_CP091582
Organism Klebsiella pneumoniae strain Nord9_R85

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag L5468_RS12700 Protein ID WP_004150910.1
Coordinates 2582555..2583040 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag L5468_RS12695 Protein ID WP_004150912.1
Coordinates 2582265..2582558 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L5468_RS12675 (L5468_12675) 2577473..2578075 - 603 WP_002916735.1 short chain dehydrogenase -
L5468_RS12680 (L5468_12680) 2578173..2579084 + 912 WP_004188550.1 LysR family transcriptional regulator -
L5468_RS12685 (L5468_12685) 2579085..2580233 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
L5468_RS12690 (L5468_12690) 2580244..2581620 - 1377 WP_004217775.1 cystathionine beta-synthase -
L5468_RS12695 (L5468_12695) 2582265..2582558 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
L5468_RS12700 (L5468_12700) 2582555..2583040 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
L5468_RS12705 (L5468_12705) 2583744..2584337 + 594 WP_004188553.1 hypothetical protein -
L5468_RS12710 (L5468_12710) 2584434..2584650 + 217 Protein_2464 transposase -
L5468_RS12715 (L5468_12715) 2585256..2586128 + 873 WP_004188557.1 ParA family protein -
L5468_RS12720 (L5468_12720) 2586128..2586511 + 384 WP_004150906.1 hypothetical protein -
L5468_RS12725 (L5468_12725) 2586504..2587871 + 1368 WP_004150905.1 SPI-7-type island replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 2584434..2584586 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T233900 WP_004150910.1 NZ_CP091582:2582555-2583040 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T233900 NZ_LN854556:963230-963337 [Staphylococcus aureus]
GTGATATCTATTGCAAATGCATTACATTTAATGTTAAGTTTCGGTATGTTTATTGTCACTTTCATTGGTGTAGTAGTCGC
AATAATTAATTTAAACAATAAAAAATAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT233900 WP_004150912.1 NZ_CP091582:2582265-2582558 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT233900 NZ_LN854556:c963446-963332 [Staphylococcus aureus]
TAAAAAGACGACATGCAGGAACATGTCGCCTATTGAGCCCGTTAAAAAGACGGTGACTAAATGAGATTTTCTTTAACCAT
CATTCGTTGTCAAAGTTTGGAAATGATGGTTATTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References