Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 898652..899427 Replicon chromosome
Accession NZ_CP063277
Organism Klebsiella pneumoniae strain MAR14-456

Toxin (Protein)


Gene name TacT2 Uniprot ID B6ZBI6
Locus tag IL253_RS04415 Protein ID WP_004889762.1
Coordinates 898942..899427 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag IL253_RS04410 Protein ID WP_004150912.1
Coordinates 898652..898945 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IL253_RS04390 893861..894463 - 603 WP_002916735.1 short chain dehydrogenase -
IL253_RS04395 894561..895472 + 912 WP_014906929.1 LysR family transcriptional regulator -
IL253_RS04400 895473..896621 - 1149 WP_014906930.1 PLP-dependent aspartate aminotransferase family protein -
IL253_RS04405 896632..898008 - 1377 WP_014906931.1 pyridoxal-phosphate dependent enzyme -
IL253_RS04410 898652..898945 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
IL253_RS04415 898942..899427 + 486 WP_004889762.1 GNAT family N-acetyltransferase Toxin
IL253_RS04420 900131..900724 + 594 WP_004188553.1 hypothetical protein -
IL253_RS04425 900821..901037 + 217 Protein_863 transposase -
IL253_RS04435 901715..902428 - 714 WP_002916694.1 DUF554 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 900821..900973 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17585.56 Da        Isoelectric Point: 8.5144

>T177846 WP_004889762.1 NZ_CP063277:898942-899427 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDSMMLMVTLRDLVN
A

Download         Length: 486 bp

>T177846 NZ_CP083728:c180177-180082 [Staphylococcus aureus]
GTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAG
ACGCAATACAAAATAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT177846 WP_004150912.1 NZ_CP063277:898652-898945 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT177846 NZ_CP083728:179995-180054 [Staphylococcus aureus]
ACAACGAAAATAAGTATTTACTTATACACCAATCCCCTCACTATTTGCGGTAGTGAGGGG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A447W563

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References