Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 987580..988355 Replicon chromosome
Accession NZ_CP065460
Organism Klebsiella pneumoniae strain Gordon

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag I5M58_RS04865 Protein ID WP_004150910.1
Coordinates 987870..988355 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag I5M58_RS04860 Protein ID WP_004150912.1
Coordinates 987580..987873 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I5M58_RS04840 982788..983390 - 603 WP_062954968.1 short chain dehydrogenase -
I5M58_RS04845 983488..984399 + 912 WP_004188550.1 LysR family transcriptional regulator -
I5M58_RS04850 984400..985548 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
I5M58_RS04855 985559..986935 - 1377 WP_004217775.1 cystathionine beta-synthase -
I5M58_RS04860 987580..987873 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
I5M58_RS04865 987870..988355 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
I5M58_RS04870 989059..989652 + 594 WP_004188553.1 hypothetical protein -
I5M58_RS04875 989749..989965 + 217 Protein_947 transposase -
I5M58_RS04880 990571..991443 + 873 WP_004188557.1 ParA family protein -
I5M58_RS04885 991443..991826 + 384 WP_004150906.1 hypothetical protein -
I5M58_RS04890 991819..993186 + 1368 WP_004150905.1 replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 989749..989901 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T182767 WP_004150910.1 NZ_CP065460:987870-988355 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T182767 NZ_CP086566:c2519931-2519830 [Enterococcus faecalis]
TTGTCAATCGAAGCGGCATTGGAATTGATGATTAGTTTTGCAGCGTTTGTTGCACTACTGATTTTCGGTATCCTTGAAGC
AACGAAAAACGATAAAAAATAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT182767 WP_004150912.1 NZ_CP065460:987580-987873 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT182767 NZ_CP086566:2519708-2519885 [Enterococcus faecalis]
AAAAGAGAGGTATGCGGGTACATACCTCTCTTTTATACCAGCGCCAGTTATAAGAACTGGTGGCTTACTGAGTTAAGTTT
TTATTACTGTTTAACCGTTCGGCCTGTCAAGCTTATGAACGGTTATTTTTTATCGTTTTTCGTTGCTTCAAGGATACCGA
AAATCAGTAGTGCAACAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References