Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 4454866..4455641 Replicon chromosome
Accession NZ_CP064174
Organism Klebsiella pneumoniae strain 19110124

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag ISU39_RS21840 Protein ID WP_004150910.1
Coordinates 4454866..4455351 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag ISU39_RS21845 Protein ID WP_004150912.1
Coordinates 4455348..4455641 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISU39_RS21815 4450035..4451402 - 1368 WP_004150905.1 replicative DNA helicase -
ISU39_RS21820 4451395..4451778 - 384 WP_004150906.1 hypothetical protein -
ISU39_RS21825 4451778..4452650 - 873 WP_004188557.1 ParA family protein -
ISU39_RS21830 4453256..4453472 - 217 Protein_4259 transposase -
ISU39_RS21835 4453569..4454162 - 594 WP_004188553.1 hypothetical protein -
ISU39_RS21840 4454866..4455351 - 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
ISU39_RS21845 4455348..4455641 - 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
ISU39_RS21850 4456286..4457662 + 1377 WP_004217775.1 cystathionine beta-synthase -
ISU39_RS21855 4457673..4458821 + 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
ISU39_RS21860 4458822..4459733 - 912 WP_004188550.1 LysR family transcriptional regulator -
ISU39_RS21865 4459831..4460433 + 603 WP_002916735.1 short chain dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Integrative and Conjugative Element - - 4397845..4455892 58047
flank IS/Tn - - 4453320..4453472 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T180420 WP_004150910.1 NZ_CP064174:c4455351-4454866 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T180420 NZ_CP085294:c210249-210148 [Enterococcus faecalis]
TTGTCAATCGAAGCGGCATTGGAATTGATGATTAGTTTTGCAGCGTTTGTTGCACTACTGATTTTCGGTATCCTTGAAGC
AACGAAAAACGATAAAAAATAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT180420 WP_004150912.1 NZ_CP064174:c4455641-4455348 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT180420 NZ_CP085294:210024-210203 [Enterococcus faecalis]
TGAAAAGAGAGATATGCTACTACATACCTCTCCTTTATACCAACACCAGTTATAAGAACTGGTGGCTTACTGAGTTAAGT
TTTTGTTGTTCTTTAACCGTTCAGCCGTCTAAGCTTATGAACGGTTATTTTTTATCGTTTTTCGTTGCTTCAAGGATACC
GAAAATCAGTAGTGCAACAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References