Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 721777..722552 Replicon chromosome
Accession NZ_CP065167
Organism Klebsiella pneumoniae strain KPN114

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A332L402
Locus tag I3U24_RS03570 Protein ID WP_021314147.1
Coordinates 722067..722552 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag I3U24_RS03565 Protein ID WP_004150912.1
Coordinates 721777..722070 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I3U24_RS03545 716985..717587 - 603 WP_021440720.1 short chain dehydrogenase -
I3U24_RS03550 717685..718596 + 912 WP_004188550.1 LysR family transcriptional regulator -
I3U24_RS03555 718597..719745 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
I3U24_RS03560 719756..721132 - 1377 WP_074194669.1 cystathionine beta-synthase -
I3U24_RS03565 721777..722070 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
I3U24_RS03570 722067..722552 + 486 WP_021314147.1 GNAT family N-acetyltransferase Toxin
I3U24_RS03575 723256..723849 + 594 WP_004188553.1 hypothetical protein -
I3U24_RS03580 723946..724162 + 217 Protein_701 transposase -
I3U24_RS03585 724937..725821 + 885 WP_013095145.1 ParA family protein -
I3U24_RS03590 725814..727178 + 1365 WP_044158999.1 replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 723946..724098 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17621.68 Da        Isoelectric Point: 8.5144

>T182205 WP_021314147.1 NZ_CP065167:722067-722552 [Klebsiella pneumoniae]
MILAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T182205 NZ_CP086340:3463603-3463821 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT182205 WP_004150912.1 NZ_CP065167:721777-722070 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT182205 NZ_CP086340:3463203-3463577 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A332L402

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References