Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 4410498..4411273 Replicon chromosome
Accession NZ_CP070564
Organism Klebsiella pneumoniae strain KP51069

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag JW299_RS23620 Protein ID WP_004150910.1
Coordinates 4410498..4410983 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag JW299_RS23625 Protein ID WP_004150912.1
Coordinates 4410980..4411273 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JW299_RS23595 4405667..4407034 - 1368 WP_004150905.1 replicative DNA helicase -
JW299_RS23600 4407027..4407410 - 384 WP_004150906.1 hypothetical protein -
JW299_RS23605 4407410..4408282 - 873 WP_004188557.1 ParA family protein -
JW299_RS23610 4408888..4409104 - 217 Protein_4254 transposase -
JW299_RS23615 4409201..4409794 - 594 WP_004188553.1 hypothetical protein -
JW299_RS23620 4410498..4410983 - 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
JW299_RS23625 4410980..4411273 - 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
JW299_RS23630 4411918..4413294 + 1377 WP_004217775.1 cystathionine beta-synthase -
JW299_RS23635 4413305..4414453 + 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
JW299_RS23640 4414454..4415365 - 912 WP_004188550.1 LysR family transcriptional regulator -
JW299_RS23645 4415463..4416065 + 603 WP_002916735.1 short chain dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Integrative and Conjugative Element - - 4353476..4411524 58048
flank IS/Tn - - 4408952..4409104 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T194347 WP_004150910.1 NZ_CP070564:c4410983-4410498 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T194347 NZ_CP093445:862199-862612 [Salmonella enterica subsp. enterica serovar Abeokuta]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCATGGCGCGCTCAGTGGATCTCATTGCTCATTCATGGGCTGGTTGCCGC
GGTGATTTTATTGATGCCCTGGCCGCTGAGTTATACCCCGTTATGGATGATACTACTGTCGCTGGTCGTGTTTGACTGTG
TACGTAGCCAGCGCAGGATTAATACCTGTCAGGGAGAGATCAAGCTACTCATGGACGGGCGCTTACGCTGGCAGGGGCAG
GACTGGACGCTCTTGCGTCCGCCCTGGTTACTGAAAAGCGGGATGGTGTTGCGTTTACGCGCAGAGTCTGGACGTCATCA
GCATTTATGGCTGGCGGCGGATAGCATGGAAGAAGCGGAATGGCGTGAGTTGCGTCGGATACTGTTACAGCAGCCGATAT
CAGGCCAACACTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT194347 WP_004150912.1 NZ_CP070564:c4411273-4410980 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT194347 NZ_CP093445:861952-862218 [Salmonella enterica subsp. enterica serovar Abeokuta]
ATGGATATTCACAATAAAGCACGTATTCACTGGGCATGTCGTCGCGGTATGCGCGAACTTGATATTTCCATCATGCCGTT
TTTTGAACATGAGTACGATAGTTTAAGTGATGAAGAAAAGCGTATTTTTGTCCGTTTGTTGCAGAGTGATGACCCGGATT
TATTTAACTGGTTAATGAATCACGGTAAACCGGCTGACGCTGAACTGGAACAGATGGTCCGACTCATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References