Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 738886..739661 Replicon chromosome
Accession NZ_CP063147
Organism Klebsiella pneumoniae strain KP19-3088

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag IMY63_RS03710 Protein ID WP_004150910.1
Coordinates 739176..739661 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag IMY63_RS03705 Protein ID WP_004150912.1
Coordinates 738886..739179 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMY63_RS03685 734094..734696 - 603 WP_002916735.1 short chain dehydrogenase -
IMY63_RS03690 734794..735705 + 912 WP_004188550.1 LysR family transcriptional regulator -
IMY63_RS03695 735706..736854 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
IMY63_RS03700 736865..738241 - 1377 WP_004217775.1 cystathionine beta-synthase -
IMY63_RS03705 738886..739179 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
IMY63_RS03710 739176..739661 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
IMY63_RS03715 740365..740958 + 594 WP_004188553.1 hypothetical protein -
IMY63_RS03720 741055..741271 + 217 Protein_727 transposase -
IMY63_RS03725 741877..742749 + 873 WP_004188557.1 ParA family protein -
IMY63_RS03730 742749..743132 + 384 WP_004150906.1 hypothetical protein -
IMY63_RS03735 743125..744492 + 1368 WP_004150905.1 replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 741055..741207 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T177600 WP_004150910.1 NZ_CP063147:739176-739661 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T177600 NZ_CP083585:c4129091-4128804 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGTATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT177600 WP_004150912.1 NZ_CP063147:738886-739179 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT177600 NZ_CP083585:c4128817-4128515 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References