Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 720988..721763 Replicon chromosome
Accession NZ_CP076453
Organism Klebsiella pneumoniae strain PM27

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A332L402
Locus tag KOM10_RS03575 Protein ID WP_021314147.1
Coordinates 721278..721763 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag KOM10_RS03570 Protein ID WP_004150912.1
Coordinates 720988..721281 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KOM10_RS03550 (KOM10_03540) 716196..716798 - 603 WP_004174410.1 short chain dehydrogenase -
KOM10_RS03555 (KOM10_03545) 716896..717807 + 912 WP_021314148.1 LysR family transcriptional regulator -
KOM10_RS03560 (KOM10_03550) 717808..718956 - 1149 WP_016531357.1 PLP-dependent aspartate aminotransferase family protein -
KOM10_RS03565 (KOM10_03555) 718967..720343 - 1377 WP_004174417.1 pyridoxal-phosphate dependent enzyme -
KOM10_RS03570 (KOM10_03560) 720988..721281 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
KOM10_RS03575 (KOM10_03565) 721278..721763 + 486 WP_021314147.1 GNAT family N-acetyltransferase Toxin
KOM10_RS03580 (KOM10_03570) 722467..723060 + 594 WP_004188553.1 hypothetical protein -
KOM10_RS03585 (KOM10_03575) 723157..723373 + 217 Protein_705 transposase -
KOM10_RS03595 (KOM10_03585) 723888..724601 - 714 WP_002916694.1 DUF554 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 723157..723309 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17621.68 Da        Isoelectric Point: 8.5144

>T205697 WP_021314147.1 NZ_CP076453:721278-721763 [Klebsiella pneumoniae]
MILAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T205697 NZ_CP101844:c1194930-1194670 [Streptococcus suis]
ATGTATCATTTAGAGTATTCTAAAAAGGCTCAAAAGCAAATTAAGAAATTAGATAGGCAAATTCAGAGGTTGTTGTTTGC
TTGGATTGATAAACACTTAGAAGGGACTGATAATCCACGCGCCAATGGTAAAGGTTTGACAGGCAATCATGCTAACGAGT
GGCGTTATCGTATCGGAGATTACCGACTGATTTGCGATATTCAAGATGATAAGTTGGTTATTTTAGCACTTGAGTTTGGA
CATCGTAAAGATGTCTATTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT205697 WP_004150912.1 NZ_CP076453:720988-721281 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT205697 NZ_CP101844:c1195153-1194932 [Streptococcus suis]
ATGAGTACAGTGACAATCAGATTAAATCAAGAAGAAGAAGTATTCTTTAAAAGCTATGCACAATTAACAGGTCAAAGTCT
TTCTAGCCTTTTTAAGAAAGCTTTGGAGCGTGATATTGAAGATGAGTATGATTTAAAAATCTATCATCAAGCTTATGATG
AGTACAAAGCAGATCCAGAAACAATTAGTCATGCTGATTTTAAGAAAGAGCTAGGATTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A332L402

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References