Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 739921..740696 Replicon chromosome
Accession NZ_CP090626
Organism Klebsiella pneumoniae isolate KpBO_TO3

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag L0104_RS03690 Protein ID WP_004150910.1
Coordinates 740211..740696 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag L0104_RS03685 Protein ID WP_004150912.1
Coordinates 739921..740214 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L0104_RS03665 (L0104_03665) 735129..735731 - 603 WP_002916735.1 short chain dehydrogenase -
L0104_RS03670 (L0104_03670) 735829..736740 + 912 WP_004188550.1 LysR family transcriptional regulator -
L0104_RS03675 (L0104_03675) 736741..737889 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
L0104_RS03680 (L0104_03680) 737900..739276 - 1377 WP_004217775.1 cystathionine beta-synthase -
L0104_RS03685 (L0104_03685) 739921..740214 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
L0104_RS03690 (L0104_03690) 740211..740696 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
L0104_RS03695 (L0104_03695) 741400..741993 + 594 WP_004188553.1 hypothetical protein -
L0104_RS03700 (L0104_03700) 742090..742306 + 217 Protein_728 transposase -
L0104_RS03705 (L0104_03705) 742912..743784 + 873 WP_004188557.1 ParA family protein -
L0104_RS03710 (L0104_03710) 743784..744167 + 384 WP_004150906.1 hypothetical protein -
L0104_RS03715 (L0104_03715) 744160..745527 + 1368 WP_004150905.1 SPI-7-type island replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 742090..742242 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T231490 WP_004150910.1 NZ_CP090626:740211-740696 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T231490 NZ_CP126627:c2430142-2429876 [Staphylococcus aureus]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACGATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGGAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT231490 WP_004150912.1 NZ_CP090626:739921-740214 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT231490 NZ_CP126627:c2430393-2430142 [Staphylococcus aureus]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATGGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAACCCAAGTAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAACGAAAGAT
ATAGATGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References