Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 759007..759782 Replicon chromosome
Accession NZ_CP046645
Organism Klebsiella pneumoniae strain EGKP3

Toxin (Protein)


Gene name TacT2 Uniprot ID B6ZBI6
Locus tag GPU01_RS03805 Protein ID WP_004889762.1
Coordinates 759297..759782 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag GPU01_RS03800 Protein ID WP_004150912.1
Coordinates 759007..759300 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GPU01_RS03780 (GPU01_03735) 754216..754818 - 603 WP_004174410.1 short chain dehydrogenase -
GPU01_RS03785 (GPU01_03740) 754916..755827 + 912 WP_017879796.1 LysR family transcriptional regulator -
GPU01_RS03790 (GPU01_03745) 755828..756976 - 1149 WP_004889759.1 PLP-dependent aspartate aminotransferase family protein -
GPU01_RS03795 (GPU01_03750) 756987..758363 - 1377 WP_017879795.1 pyridoxal-phosphate dependent enzyme -
GPU01_RS03800 (GPU01_03755) 759007..759300 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
GPU01_RS03805 (GPU01_03760) 759297..759782 + 486 WP_004889762.1 GNAT family N-acetyltransferase Toxin
GPU01_RS03810 (GPU01_03765) 760486..761078 + 593 Protein_750 hypothetical protein -
GPU01_RS03815 (GPU01_03770) 761175..761391 + 217 Protein_751 transposase -
GPU01_RS03825 (GPU01_03785) 761906..762619 - 714 WP_002916694.1 DUF554 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 761175..761327 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17585.56 Da        Isoelectric Point: 8.5144

>T144637 WP_004889762.1 NZ_CP046645:759297-759782 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDSMMLMVTLRDLVN
A

Download         Length: 486 bp

>T144637 NZ_CP062204:4549769-4550146 [Escherichia coli]
ATGAATACATTACCCGACACTCACGTACGGGAGACATCGGGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCCCGCCGGGCAACCGGCCTGATGACCCGCAG
CCACTACAGAACGGTAAATGACATTACCCTGGGTAAACGTACGGAGGCAAAACGATGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT144637 WP_004150912.1 NZ_CP046645:759007-759300 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT144637 NZ_CP062204:4549345-4549722 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCCCGACTGGTACAGGAGGGTAACCGCCTGCATTACCTTGCAGACCGCGCCAGTATCAGAGGCAGGT
TCAGCGACGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCGGCGGT
GAACTGAATCCCCGTCATCAGCATACCGTCACACTGTATGCGAAAGGGCTGACCTGCGAAGCCGATACCCTTGGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCGGCAGCGCCCGCAATCACCGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A447W563

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References