Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 743843..744618 Replicon chromosome
Accession NZ_CP073302
Organism Klebsiella pneumoniae strain SW1780

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag KCX81_RS03705 Protein ID WP_004150910.1
Coordinates 744133..744618 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag KCX81_RS03700 Protein ID WP_004150912.1
Coordinates 743843..744136 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KCX81_RS03680 (KCX81_03680) 739051..739653 - 603 WP_062954968.1 short chain dehydrogenase -
KCX81_RS03685 (KCX81_03685) 739751..740662 + 912 WP_004188550.1 LysR family transcriptional regulator -
KCX81_RS03690 (KCX81_03690) 740663..741811 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
KCX81_RS03695 (KCX81_03695) 741822..743198 - 1377 WP_004217775.1 cystathionine beta-synthase -
KCX81_RS03700 (KCX81_03700) 743843..744136 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
KCX81_RS03705 (KCX81_03705) 744133..744618 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
KCX81_RS03710 (KCX81_03710) 745322..745915 + 594 WP_004188553.1 hypothetical protein -
KCX81_RS03715 (KCX81_03715) 746012..746228 + 217 Protein_731 transposase -
KCX81_RS03720 (KCX81_03720) 746834..747706 + 873 WP_004188557.1 ParA family protein -
KCX81_RS03725 (KCX81_03725) 747706..748089 + 384 WP_004150906.1 hypothetical protein -
KCX81_RS03730 (KCX81_03730) 748082..749449 + 1368 WP_004150905.1 SPI-7-type island replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 746012..746164 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T199668 WP_004150910.1 NZ_CP073302:744133-744618 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T199668 NZ_CP097467:478466-478816 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT199668 WP_004150912.1 NZ_CP073302:743843-744136 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT199668 NZ_CP097467:478179-478460 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References