Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 770664..771439 Replicon chromosome
Accession NZ_CP049716
Organism Klebsiella pneumoniae strain 11KM256

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag G7Z27_RS03900 Protein ID WP_004150910.1
Coordinates 770954..771439 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag G7Z27_RS03895 Protein ID WP_004150912.1
Coordinates 770664..770957 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G7Z27_RS03875 765872..766474 - 603 WP_002916735.1 short chain dehydrogenase -
G7Z27_RS03880 766572..767483 + 912 WP_004188550.1 LysR family transcriptional regulator -
G7Z27_RS03885 767484..768632 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
G7Z27_RS03890 768643..770019 - 1377 WP_004217775.1 cystathionine beta-synthase -
G7Z27_RS03895 770664..770957 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
G7Z27_RS03900 770954..771439 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
G7Z27_RS03905 772143..772736 + 594 WP_004188553.1 hypothetical protein -
G7Z27_RS03910 772833..773049 + 217 Protein_769 transposase -
G7Z27_RS03915 773655..774527 + 873 WP_004188557.1 ParA family protein -
G7Z27_RS03920 774527..774910 + 384 WP_004150906.1 hypothetical protein -
G7Z27_RS03925 774903..776270 + 1368 WP_004150905.1 replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 772833..772985 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T151437 WP_004150910.1 NZ_CP049716:770954-771439 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T151437 NZ_CP065472:c54305-54147 [Klebsiella quasipneumoniae]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT151437 WP_004150912.1 NZ_CP049716:770664-770957 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT151437 NZ_CP065472:c54573-54349 [Klebsiella quasipneumoniae]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References