Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 741275..742050 Replicon chromosome
Accession NZ_CP092759
Organism Klebsiella pneumoniae strain bat16

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag MKZ45_RS03700 Protein ID WP_004150910.1
Coordinates 741565..742050 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag MKZ45_RS03695 Protein ID WP_004150912.1
Coordinates 741275..741568 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MKZ45_RS03675 (MKZ45_03675) 736483..737085 - 603 WP_002916735.1 short chain dehydrogenase -
MKZ45_RS03680 (MKZ45_03680) 737183..738094 + 912 WP_004188550.1 LysR family transcriptional regulator -
MKZ45_RS03685 (MKZ45_03685) 738095..739243 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
MKZ45_RS03690 (MKZ45_03690) 739254..740630 - 1377 WP_004217775.1 cystathionine beta-synthase -
MKZ45_RS03695 (MKZ45_03695) 741275..741568 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
MKZ45_RS03700 (MKZ45_03700) 741565..742050 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
MKZ45_RS03705 (MKZ45_03705) 742754..743347 + 594 WP_004188553.1 hypothetical protein -
MKZ45_RS03710 (MKZ45_03710) 743444..743660 + 217 Protein_729 transposase -
MKZ45_RS03715 (MKZ45_03715) 744103..744975 + 873 WP_154717906.1 ParA family protein -
MKZ45_RS03720 (MKZ45_03720) 744975..745358 + 384 WP_004150906.1 hypothetical protein -
MKZ45_RS03725 (MKZ45_03725) 745351..746718 + 1368 WP_004150905.1 SPI-7-type island replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 743444..743596 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T237089 WP_004150910.1 NZ_CP092759:741565-742050 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T237089 NZ_LR778150:354922-355197 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATAGTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGCAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT237089 WP_004150912.1 NZ_CP092759:741275-741568 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT237089 NZ_LR778150:355194-355553 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References