Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 742973..743748 Replicon chromosome
Accession NZ_CP043353
Organism Klebsiella pneumoniae strain WCHKP020115

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag C6M09_RS03720 Protein ID WP_004150910.1
Coordinates 743263..743748 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag C6M09_RS03715 Protein ID WP_004150912.1
Coordinates 742973..743266 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6M09_RS03695 (C6M09_03695) 738181..738783 - 603 WP_062954968.1 short chain dehydrogenase -
C6M09_RS03700 (C6M09_03700) 738881..739792 + 912 WP_004188550.1 LysR family transcriptional regulator -
C6M09_RS03705 (C6M09_03705) 739793..740941 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
C6M09_RS03710 (C6M09_03710) 740952..742328 - 1377 WP_004217775.1 cystathionine beta-synthase -
C6M09_RS03715 (C6M09_03715) 742973..743266 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
C6M09_RS03720 (C6M09_03720) 743263..743748 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
C6M09_RS03725 (C6M09_03725) 744452..745045 + 594 WP_004188553.1 hypothetical protein -
C6M09_RS03730 (C6M09_03730) 745142..745358 + 217 Protein_734 transposase -
C6M09_RS03735 (C6M09_03735) 745964..746836 + 873 WP_004188557.1 ParA family protein -
C6M09_RS03740 (C6M09_03740) 746836..747219 + 384 WP_004150906.1 hypothetical protein -
C6M09_RS03745 (C6M09_03745) 747212..748579 + 1368 WP_004150905.1 SPI-7-type island replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 745142..745294 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T138466 WP_004150910.1 NZ_CP043353:743263-743748 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T138466 NZ_CP058057:c438284-437820 [Escherichia fergusonii]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTACAGCGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAGCCCATTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT138466 WP_004150912.1 NZ_CP043353:742973-743266 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT138466 NZ_CP043353:742973-743266 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References