Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 741121..741896 Replicon chromosome
Accession NZ_CP092717
Organism Klebsiella pneumoniae strain HvKp-su1

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag MKX18_RS03705 Protein ID WP_004150910.1
Coordinates 741411..741896 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag MKX18_RS03700 Protein ID WP_004150912.1
Coordinates 741121..741414 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MKX18_RS03680 (MKX18_03680) 736329..736931 - 603 WP_062954968.1 short chain dehydrogenase -
MKX18_RS03685 (MKX18_03685) 737029..737940 + 912 WP_004188550.1 LysR family transcriptional regulator -
MKX18_RS03690 (MKX18_03690) 737941..739089 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
MKX18_RS03695 (MKX18_03695) 739100..740476 - 1377 WP_004217775.1 cystathionine beta-synthase -
MKX18_RS03700 (MKX18_03700) 741121..741414 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
MKX18_RS03705 (MKX18_03705) 741411..741896 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
MKX18_RS03710 (MKX18_03710) 742600..743193 + 594 WP_004188553.1 hypothetical protein -
MKX18_RS03715 (MKX18_03715) 743290..743506 + 217 Protein_730 transposase -
MKX18_RS03720 (MKX18_03720) 744112..744984 + 873 WP_004188557.1 ParA family protein -
MKX18_RS03725 (MKX18_03725) 744984..745367 + 384 WP_004150906.1 hypothetical protein -
MKX18_RS03730 (MKX18_03730) 745360..746727 + 1368 WP_004150905.1 SPI-7-type island replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 743290..743442 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T237024 WP_004150910.1 NZ_CP092717:741411-741896 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T237024 NZ_LR778146:3677310-3677417 [Escherichia coli]
ATGACGCTCGCAGAACTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGCATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT237024 WP_004150912.1 NZ_CP092717:741121-741414 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT237024 NZ_LR778146:c3677262-3677196 [Escherichia coli]
GGTCTAGGTTCAAGATTAGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References