Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 733978..734753 Replicon chromosome
Accession NZ_CP089024
Organism Klebsiella pneumoniae strain 49210

Toxin (Protein)


Gene name TacT2 Uniprot ID B6ZBI6
Locus tag LRY78_RS03635 Protein ID WP_004889762.1
Coordinates 734268..734753 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag LRY78_RS03630 Protein ID WP_004150912.1
Coordinates 733978..734271 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LRY78_RS03610 (729187) 729187..729789 - 603 WP_002916735.1 short chain dehydrogenase -
LRY78_RS03615 (729887) 729887..730798 + 912 WP_014906929.1 LysR family transcriptional regulator -
LRY78_RS03620 (730799) 730799..731947 - 1149 WP_014906930.1 PLP-dependent aspartate aminotransferase family protein -
LRY78_RS03625 (731958) 731958..733334 - 1377 WP_014906931.1 pyridoxal-phosphate dependent enzyme -
LRY78_RS03630 (733978) 733978..734271 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
LRY78_RS03635 (734268) 734268..734753 + 486 WP_004889762.1 GNAT family N-acetyltransferase Toxin
LRY78_RS03640 (735457) 735457..736050 + 594 WP_004188553.1 hypothetical protein -
LRY78_RS03645 (736147) 736147..736363 + 217 Protein_717 transposase -
LRY78_RS03655 (737041) 737041..737754 - 714 WP_002916694.1 DUF554 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 736147..736299 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17585.56 Da        Isoelectric Point: 8.5144

>T227407 WP_004889762.1 NZ_CP089024:734268-734753 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDSMMLMVTLRDLVN
A

Download         Length: 486 bp

>T227407 NZ_CP122868:2087280-2087383 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT227407 WP_004150912.1 NZ_CP089024:733978-734271 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT227407 NZ_CP122868:c2087421-2087150 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A447W563

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References