Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 1288968..1289743 Replicon chromosome
Accession NZ_CP008797
Organism Klebsiella pneumoniae subsp. pneumoniae KPNIH24

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag KPNIH24_RS06475 Protein ID WP_004150910.1
Coordinates 1289258..1289743 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag KPNIH24_RS06470 Protein ID WP_004150912.1
Coordinates 1288968..1289261 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KPNIH24_RS06450 1284176..1284778 - 603 WP_002916735.1 short chain dehydrogenase -
KPNIH24_RS06455 1284876..1285787 + 912 WP_004188550.1 LysR family transcriptional regulator -
KPNIH24_RS06460 1285788..1286936 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
KPNIH24_RS06465 1286947..1288323 - 1377 WP_004217775.1 cystathionine beta-synthase -
KPNIH24_RS06470 1288968..1289261 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
KPNIH24_RS06475 1289258..1289743 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
KPNIH24_RS06480 1290447..1291040 + 594 WP_004188553.1 hypothetical protein -
KPNIH24_RS06485 1291137..1291353 + 217 Protein_1194 transposase -
KPNIH24_RS06490 1291959..1292831 + 873 WP_004188557.1 ParA family protein -
KPNIH24_RS06495 1292831..1293214 + 384 WP_004150906.1 hypothetical protein -
KPNIH24_RS06500 1293207..1294574 + 1368 WP_004150905.1 replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 1291137..1291289 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T47471 WP_004150910.1 NZ_CP008797:1289258-1289743 [Klebsiella pneumoniae subsp. pneumoniae KPNIH24]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T47471 NZ_CP008797:1289258-1289743 [Klebsiella pneumoniae subsp. pneumoniae KPNIH24]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT47471 WP_004150912.1 NZ_CP008797:1288968-1289261 [Klebsiella pneumoniae subsp. pneumoniae KPNIH24]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT47471 NZ_CP008797:1288968-1289261 [Klebsiella pneumoniae subsp. pneumoniae KPNIH24]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References