Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 709557..710332 Replicon chromosome
Accession NZ_CP081896
Organism Klebsiella pneumoniae strain PA-01

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A332L402
Locus tag KZ665_RS03495 Protein ID WP_021314147.1
Coordinates 709847..710332 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag KZ665_RS03490 Protein ID WP_004150912.1
Coordinates 709557..709850 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZ665_RS03470 (KZ665_03470) 704765..705367 - 603 WP_032448122.1 short chain dehydrogenase -
KZ665_RS03475 (KZ665_03475) 705465..706376 + 912 WP_021314148.1 LysR family transcriptional regulator -
KZ665_RS03480 (KZ665_03480) 706377..707525 - 1149 WP_064179254.1 PLP-dependent aspartate aminotransferase family protein -
KZ665_RS03485 (KZ665_03485) 707536..708912 - 1377 WP_004174417.1 pyridoxal-phosphate dependent enzyme -
KZ665_RS03490 (KZ665_03490) 709557..709850 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
KZ665_RS03495 (KZ665_03495) 709847..710332 + 486 WP_021314147.1 GNAT family N-acetyltransferase Toxin
KZ665_RS03500 (KZ665_03500) 711036..711674 + 639 Protein_688 hypothetical protein -
KZ665_RS03505 (KZ665_03505) 711722..711938 + 217 Protein_689 transposase -
KZ665_RS03515 (KZ665_03515) 712616..713329 - 714 WP_002916694.1 DUF554 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 711722..711874 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17621.68 Da        Isoelectric Point: 8.5144

>T213761 WP_021314147.1 NZ_CP081896:709847-710332 [Klebsiella pneumoniae]
MILAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T213761 NZ_CP109846:3071655-3071963 [Providencia rettgeri]
ATGCAATATTGCCTCTATCAAAATCGAGAAGATACAGTCAAACACCCATATTTACTGGACATTCAAAGTAATATTATTGA
TATATTGAATACTCTTTTAGTCATCCCGTTATTCGATAGCAGGCTAGCTAAAAAACCATTACCTACTTGCCTTAACCCCC
AGTTATTTAGCAACGGGCAGGTGTTTGTTTTAATGACACACCAAATGGCTTGCGTCCCTCATTCATTATTAGGGAAAGAA
ATCGTTGACTTGAACTGCCAACAAAACACGATTAAACACGCAATTGACTTACTTATTGATGGCTTTTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT213761 WP_004150912.1 NZ_CP081896:709557-709850 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT213761 NZ_CP109846:3071455-3071655 [Providencia rettgeri]
ATCACTGTAGCGCCTGAGCTCTGCGAACAAGCAAGGCGGGCTAAACTCAACTTATCTGCAATACTAACTTGTGCCTTACA
AGCTGAATTAAAACGCCTAGAAGCACAAAAGTGGAAAAATGAAAATATCGAAGGTTTTAATCAATTAAATAGGAACAATG
AAGAATACGGATTACTCTCAGATAAATACAAGGGTTTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A332L402

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References