Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 742644..743419 Replicon chromosome
Accession NZ_CP085889
Organism Klebsiella pneumoniae strain 8695

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag LKW25_RS03705 Protein ID WP_004150910.1
Coordinates 742934..743419 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag LKW25_RS03700 Protein ID WP_004150912.1
Coordinates 742644..742937 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LKW25_RS03680 (LKW25_03680) 737852..738454 - 603 WP_062954968.1 short chain dehydrogenase -
LKW25_RS03685 (LKW25_03685) 738552..739463 + 912 WP_004188550.1 LysR family transcriptional regulator -
LKW25_RS03690 (LKW25_03690) 739464..740612 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
LKW25_RS03695 (LKW25_03695) 740623..741999 - 1377 WP_004217775.1 cystathionine beta-synthase -
LKW25_RS03700 (LKW25_03700) 742644..742937 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
LKW25_RS03705 (LKW25_03705) 742934..743419 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
LKW25_RS03710 (LKW25_03710) 744123..744716 + 594 WP_004188553.1 hypothetical protein -
LKW25_RS03715 (LKW25_03715) 744813..745029 + 217 Protein_730 transposase -
LKW25_RS03720 (LKW25_03720) 745635..746507 + 873 WP_004188557.1 ParA family protein -
LKW25_RS03725 (LKW25_03725) 746507..746890 + 384 WP_004150906.1 hypothetical protein -
LKW25_RS03730 (LKW25_03730) 746883..748250 + 1368 WP_004150905.1 SPI-7-type island replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 744813..744965 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T222239 WP_004150910.1 NZ_CP085889:742934-743419 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T222239 NZ_CP117730:c4819035-4818625 [Klebsiella grimontii]
GTGGTCCTGTGGCAATCTGATTTACGCATCTCGTGGCGCGCGCAGTGGTTTTCACTGCTGATGCACGGCGTGGTGGCGGC
GCTGGTGCTGCTGATGCCCTGGCCGCTGAGCTATACCCCGCTGTGGCTGATACTGCTGTCGCTGGTAGTGTTCGACTGCG
TACGCAGCCAGCGGCGGATCCACGCCCGTCAGGGCGAAATAAAGCTGCTGATCGATTCGCGTCTGCGCTGGCAAAAGGCG
GAGTGGGAGATTATCGGTACGCCGTGGGTAATCAACAGCGGCATGCTGCTGCGCCTGCGGAATACGGAAAACCAACGTAC
CCAGCATTTATGGGTCGCGGCGGATAGCATGGACGCGGGAGAATGGCGCGACCTGCGTCGGCTGGTGTTACAGAAACCGA
CGCAAGAGTAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT222239 WP_004150912.1 NZ_CP085889:742644-742937 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT222239 NZ_CP117730:c4819282-4819016 [Klebsiella grimontii]
ATGGACATTAACAATAAAGCCCGTATCCATTGGGCATGCCGTCGAGGTATGCGTGAACTCGATATCTCCATCATGCCGTT
TTTCGAGCATGAGTACGATACGCTAAGCGATGGCGATAAGGCGTTATTCATCCGCCTGCTGGAAAACGACGATCCGGATC
TCTTCAACTGGTTGATGAATCATGGCGAGCCGGCTGATGCTGAACTGCAGCGGATGGTAAAATTAATTCAGACTCGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References