Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 715191..715966 Replicon chromosome
Accession NZ_CP043585
Organism Klebsiella pneumoniae subsp. ozaenae strain SCKP020151

Toxin (Protein)


Gene name TacT2 Uniprot ID B6ZBI6
Locus tag CLQ49_RS03540 Protein ID WP_004889762.1
Coordinates 715481..715966 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag CLQ49_RS03535 Protein ID WP_004150912.1
Coordinates 715191..715484 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CLQ49_RS03515 (CLQ49_03525) 710400..711002 - 603 WP_004174410.1 short chain dehydrogenase -
CLQ49_RS03520 (CLQ49_03530) 711100..712011 + 912 WP_017879796.1 LysR family transcriptional regulator -
CLQ49_RS03525 (CLQ49_03535) 712012..713160 - 1149 WP_004889759.1 PLP-dependent aspartate aminotransferase family protein -
CLQ49_RS03530 (CLQ49_03540) 713171..714547 - 1377 WP_017879795.1 pyridoxal-phosphate dependent enzyme -
CLQ49_RS03535 (CLQ49_03545) 715191..715484 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
CLQ49_RS03540 (CLQ49_03550) 715481..715966 + 486 WP_004889762.1 GNAT family N-acetyltransferase Toxin
CLQ49_RS03545 (CLQ49_03555) 716670..717262 + 593 Protein_697 hypothetical protein -
CLQ49_RS03550 (CLQ49_03560) 717359..717575 + 217 Protein_698 transposase -
CLQ49_RS03560 (CLQ49_03575) 718090..718803 - 714 WP_002916694.1 DUF554 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 717359..717511 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17585.56 Da        Isoelectric Point: 8.5144

>T139076 WP_004889762.1 NZ_CP043585:715481-715966 [Klebsiella pneumoniae subsp. ozaenae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDSMMLMVTLRDLVN
A

Download         Length: 486 bp

>T139076 NZ_CP043585:715481-715966 [Klebsiella pneumoniae subsp. ozaenae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGACCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCGCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATTCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT139076 WP_004150912.1 NZ_CP043585:715191-715484 [Klebsiella pneumoniae subsp. ozaenae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT139076 NZ_CP058350:c2543757-2543503 [Rhizobium sp. ADMK78]
ATGAGAACCACATCCTACAGCGACCTGCGCAAGAACCTGGCCTCCCTGCTCGACAGCGTGACGGACGATCGGACGCCCGT
GCTGATCACCCGTGATCGCGGCAAGCCTGCCGCCGTTCTGATGTCGGTGGAGGATTTCGCCTCCTATGAGGAAACACAGC
ACCTATTGAAGAGCCCCCGCAACGCGGAACGCCTGCTTGAAGCCGTGCGCGATCTCGACGAAGGCAAGGGCGACGTGCGC
GAGCTTTCGGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A447W563

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References