Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 3894530..3895305 Replicon chromosome
Accession NZ_CP047336
Organism Klebsiella pneumoniae strain 2019036D

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag GTH21_RS19575 Protein ID WP_004150910.1
Coordinates 3894530..3895015 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag GTH21_RS19580 Protein ID WP_004150912.1
Coordinates 3895012..3895305 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GTH21_RS19550 3889699..3891066 - 1368 WP_004150905.1 replicative DNA helicase -
GTH21_RS19555 3891059..3891442 - 384 WP_004150906.1 hypothetical protein -
GTH21_RS19560 3891442..3892314 - 873 WP_004188557.1 ParA family protein -
GTH21_RS19565 3892920..3893136 - 217 Protein_3744 transposase -
GTH21_RS19570 3893233..3893826 - 594 WP_004188553.1 hypothetical protein -
GTH21_RS19575 3894530..3895015 - 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
GTH21_RS19580 3895012..3895305 - 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
GTH21_RS19585 3895950..3897326 + 1377 WP_004217775.1 cystathionine beta-synthase -
GTH21_RS19590 3897337..3898485 + 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
GTH21_RS19595 3898486..3899397 - 912 WP_004188550.1 LysR family transcriptional regulator -
GTH21_RS19600 3899495..3900097 + 603 WP_002916735.1 short chain dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Integrative and Conjugative Element - - 3836310..3895556 59246
flank IS/Tn - - 3892984..3893136 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T145997 WP_004150910.1 NZ_CP047336:c3895015-3894530 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T145997 NZ_CP062715:c3386564-3386190 [Escherichia coli O157:H7]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTTAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACCTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT145997 WP_004150912.1 NZ_CP047336:c3895305-3895012 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT145997 NZ_CP062715:c3386985-3386611 [Escherichia coli O157:H7]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGATATCAGCATACCGTCACGCTGAATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCAGCACCCGCAACCACCTCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References