Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 716321..717096 Replicon chromosome
Accession NZ_CP055174
Organism Klebsiella pneumoniae strain STEFF_19

Toxin (Protein)


Gene name TacT2 Uniprot ID B6ZBI6
Locus tag HUZ47_RS03545 Protein ID WP_004889762.1
Coordinates 716611..717096 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag HUZ47_RS03540 Protein ID WP_004150912.1
Coordinates 716321..716614 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ47_RS03520 (HUZ47_03515) 711530..712132 - 603 WP_004174410.1 short chain dehydrogenase -
HUZ47_RS03525 (HUZ47_03520) 712230..713141 + 912 WP_017879796.1 LysR family transcriptional regulator -
HUZ47_RS03530 (HUZ47_03525) 713142..714290 - 1149 WP_004889759.1 PLP-dependent aspartate aminotransferase family protein -
HUZ47_RS03535 (HUZ47_03530) 714301..715677 - 1377 WP_017879795.1 pyridoxal-phosphate dependent enzyme -
HUZ47_RS03540 (HUZ47_03535) 716321..716614 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
HUZ47_RS03545 (HUZ47_03540) 716611..717096 + 486 WP_004889762.1 GNAT family N-acetyltransferase Toxin
HUZ47_RS03550 (HUZ47_03545) 717800..718392 + 593 Protein_698 hypothetical protein -
HUZ47_RS03555 (HUZ47_03550) 718489..718705 + 217 Protein_699 transposase -
HUZ47_RS03565 (HUZ47_03560) 719220..719933 - 714 WP_002916694.1 DUF554 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 718489..718641 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17585.56 Da        Isoelectric Point: 8.5144

>T161739 WP_004889762.1 NZ_CP055174:716611-717096 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDSMMLMVTLRDLVN
A

Download         Length: 486 bp

>T161739 NZ_CP071261:c5184973-5184590 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT161739 WP_004150912.1 NZ_CP055174:716321-716614 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT161739 NZ_CP071261:c5185215-5184973 [Klebsiella pneumoniae]
ATGATGGCTGAAAAGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A447W563

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References