Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 1439980..1440755 Replicon chromosome
Accession NZ_CP027612
Organism Klebsiella pneumoniae subsp. ozaenae strain AR_0096

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag AM475_RS07240 Protein ID WP_004150910.1
Coordinates 1439980..1440465 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag AM475_RS07245 Protein ID WP_004150912.1
Coordinates 1440462..1440755 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM475_RS07215 1436979..1437692 + 714 WP_002916694.1 DUF554 domain-containing protein -
AM475_RS07230 1438370..1438586 - 217 Protein_1379 transposase -
AM475_RS07235 1438683..1439276 - 594 WP_004188553.1 hypothetical protein -
AM475_RS07240 1439980..1440465 - 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
AM475_RS07245 1440462..1440755 - 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
AM475_RS07250 1441400..1442776 + 1377 WP_004217775.1 cystathionine beta-synthase -
AM475_RS07255 1442787..1443935 + 1149 WP_106475434.1 PLP-dependent aspartate aminotransferase family protein -
AM475_RS07260 1443936..1444847 - 912 WP_004188550.1 LysR family transcriptional regulator -
AM475_RS07265 1444945..1445547 + 603 WP_002916735.1 short chain dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 1438434..1438586 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T99773 WP_004150910.1 NZ_CP027612:c1440465-1439980 [Klebsiella pneumoniae subsp. ozaenae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T99773 NZ_CP027612:c1440465-1439980 [Klebsiella pneumoniae subsp. ozaenae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT99773 WP_004150912.1 NZ_CP027612:c1440755-1440462 [Klebsiella pneumoniae subsp. ozaenae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT99773 NZ_CP027612:c1440755-1440462 [Klebsiella pneumoniae subsp. ozaenae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References