Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 4621344..4622119 Replicon chromosome
Accession NZ_CP014647
Organism Klebsiella pneumoniae strain KPNIH36

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag ABA76_RS23375 Protein ID WP_004150910.1
Coordinates 4621344..4621829 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag ABA76_RS23380 Protein ID WP_004150912.1
Coordinates 4621826..4622119 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ABA76_RS23350 4616513..4617880 - 1368 WP_004150905.1 replicative DNA helicase -
ABA76_RS23355 4617873..4618256 - 384 WP_004150906.1 hypothetical protein -
ABA76_RS23360 4618256..4619128 - 873 WP_004188557.1 ParA family protein -
ABA76_RS23365 4619734..4619950 - 217 Protein_4449 transposase -
ABA76_RS23370 4620047..4620640 - 594 WP_004188553.1 hypothetical protein -
ABA76_RS23375 4621344..4621829 - 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
ABA76_RS23380 4621826..4622119 - 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
ABA76_RS23385 4622764..4624140 + 1377 WP_004217775.1 cystathionine beta-synthase -
ABA76_RS23390 4624151..4625299 + 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
ABA76_RS23395 4625300..4626211 - 912 WP_004188550.1 LysR family transcriptional regulator -
ABA76_RS23400 4626309..4626911 + 603 WP_002916735.1 short chain dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Integrative and Conjugative Element - - 4563123..4622370 59247
flank IS/Tn - - 4619798..4619950 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T61637 WP_004150910.1 NZ_CP014647:c4621829-4621344 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T61637 NZ_CP014647:c4621829-4621344 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT61637 WP_004150912.1 NZ_CP014647:c4622119-4621826 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT61637 NZ_CP014647:c4622119-4621826 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References