166567

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 4550369..4551144 Replicon chromosome
Accession NZ_CP058325
Organism Klebsiella pneumoniae subsp. pneumoniae strain LC-1825/18

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag GLO21_RS22430 Protein ID WP_004150910.1
Coordinates 4550369..4550854 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag GLO21_RS22435 Protein ID WP_004150912.1
Coordinates 4550851..4551144 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GLO21_RS22405 (GLO21_022400) 4545538..4546905 - 1368 WP_004150905.1 SPI-7-type island replicative DNA helicase -
GLO21_RS22410 (GLO21_022405) 4546898..4547281 - 384 WP_004150906.1 hypothetical protein -
GLO21_RS22415 (GLO21_022410) 4547281..4548153 - 873 WP_004188557.1 ParA family protein -
GLO21_RS22420 (GLO21_022415) 4548759..4548975 - 217 Protein_4391 transposase -
GLO21_RS22425 (GLO21_022420) 4549072..4549665 - 594 WP_004188553.1 hypothetical protein -
GLO21_RS22430 (GLO21_022425) 4550369..4550854 - 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
GLO21_RS22435 (GLO21_022430) 4550851..4551144 - 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
GLO21_RS22440 (GLO21_022435) 4551789..4553165 + 1377 WP_004217775.1 cystathionine beta-synthase -
GLO21_RS22445 (GLO21_022440) 4553176..4554324 + 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
GLO21_RS22450 (GLO21_022445) 4554325..4555236 - 912 WP_004188550.1 LysR family transcriptional regulator -
GLO21_RS22455 (GLO21_022450) 4555334..4555936 + 603 WP_002916735.1 short chain dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Integrative and Conjugative Element - - 4493348..4551395 58047
flank IS/Tn - - 4548823..4548975 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T166567 WP_004150910.1 NZ_CP058325:c4550854-4550369 [Klebsiella pneumoniae subsp. pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T166567 NZ_CP074354:989882-990217 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAAGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCTTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACACTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT166567 WP_004150912.1 NZ_CP058325:c4551144-4550851 [Klebsiella pneumoniae subsp. pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT166567 NZ_CP074354:989634-989882 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATTCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGGCATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References