Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 4057443..4058218 Replicon chromosome
Accession NZ_CP102435
Organism Klebsiella pneumoniae strain K194

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag NR799_RS20035 Protein ID WP_004150910.1
Coordinates 4057443..4057928 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag NR799_RS20040 Protein ID WP_004150912.1
Coordinates 4057925..4058218 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NR799_RS20005 (NR799_20005) 4052772..4053155 - 384 WP_004150906.1 hypothetical protein -
NR799_RS20010 (NR799_20010) 4053155..4054027 - 873 WP_004188557.1 ParA family protein -
NR799_RS20015 (NR799_20015) 4054633..4054849 - 217 Protein_3923 transposase -
NR799_RS20020 (NR799_20020) 4054946..4055035 - 90 Protein_3924 hypothetical protein -
NR799_RS20025 (NR799_20025) 4055185..4056165 - 981 WP_000019445.1 IS5-like element ISKpn26 family transposase -
NR799_RS20030 (NR799_20030) 4056230..4056739 - 510 Protein_3926 hypothetical protein -
NR799_RS20035 (NR799_20035) 4057443..4057928 - 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
NR799_RS20040 (NR799_20040) 4057925..4058218 - 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
NR799_RS20045 (NR799_20045) 4058863..4060239 + 1377 WP_004217775.1 cystathionine beta-synthase -
NR799_RS20050 (NR799_20050) 4060250..4061398 + 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
NR799_RS20055 (NR799_20055) 4061399..4062310 - 912 WP_004188550.1 LysR family transcriptional regulator -
NR799_RS20060 (NR799_20060) 4062408..4063010 + 603 WP_062954968.1 short chain dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Integrative and Conjugative Element - - 3994825..4058469 63644


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T253639 WP_004150910.1 NZ_CP102435:c4057928-4057443 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT253639 WP_004150912.1 NZ_CP102435:c4058218-4057925 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References