Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 5456305..5457080 Replicon chromosome
Accession NZ_CP089762
Organism Klebsiella pneumoniae strain HKC1

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag LVO51_RS27050 Protein ID WP_004150910.1
Coordinates 5456595..5457080 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag LVO51_RS27045 Protein ID WP_004150912.1
Coordinates 5456305..5456598 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVO51_RS27025 (LVO51_26990) 5451513..5452115 - 603 WP_062954968.1 short chain dehydrogenase -
LVO51_RS27030 (LVO51_26995) 5452213..5453124 + 912 WP_004188550.1 LysR family transcriptional regulator -
LVO51_RS27035 (LVO51_27000) 5453125..5454273 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
LVO51_RS27040 (LVO51_27005) 5454284..5455660 - 1377 WP_004217775.1 cystathionine beta-synthase -
LVO51_RS27045 (LVO51_27010) 5456305..5456598 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
LVO51_RS27050 (LVO51_27015) 5456595..5457080 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
LVO51_RS27055 (LVO51_27020) 5457784..5458377 + 594 WP_004188553.1 hypothetical protein -
LVO51_RS27060 (LVO51_27025) 5458474..5458690 + 217 Protein_5290 transposase -
LVO51_RS27065 (LVO51_27030) 5459296..5460168 + 873 WP_004188557.1 ParA family protein -
LVO51_RS27070 (LVO51_27035) 5460168..5460551 + 384 WP_004150906.1 hypothetical protein -
LVO51_RS27075 (LVO51_27040) 5460544..5461911 + 1368 WP_004150905.1 SPI-7-type island replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 5458474..5458626 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T228595 WP_004150910.1 NZ_CP089762:5456595-5457080 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T228595 NZ_CP124326:188541-188648 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGTTCCGGTCATTACTGGCATTCTTGCCAGTATGAT
CGTGAGCTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT228595 WP_004150912.1 NZ_CP089762:5456305-5456598 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT228595 NZ_CP124326:c188484-188427 [Escherichia coli]
TCAAGATTAGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References