Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 729498..730273 Replicon chromosome
Accession NZ_CP084866
Organism Klebsiella pneumoniae strain SA12

Toxin (Protein)


Gene name TacT2 Uniprot ID B6ZBI6
Locus tag LGM09_RS03610 Protein ID WP_004889762.1
Coordinates 729788..730273 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag LGM09_RS03605 Protein ID WP_004150912.1
Coordinates 729498..729791 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LGM09_RS03585 (724707) 724707..725309 - 603 WP_002916735.1 short chain dehydrogenase -
LGM09_RS03590 (725407) 725407..726318 + 912 WP_014906929.1 LysR family transcriptional regulator -
LGM09_RS03595 (726319) 726319..727467 - 1149 WP_014906930.1 PLP-dependent aspartate aminotransferase family protein -
LGM09_RS03600 (727478) 727478..728854 - 1377 WP_014906931.1 pyridoxal-phosphate dependent enzyme -
LGM09_RS03605 (729498) 729498..729791 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
LGM09_RS03610 (729788) 729788..730273 + 486 WP_004889762.1 GNAT family N-acetyltransferase Toxin
LGM09_RS03615 (730977) 730977..731570 + 594 WP_004188553.1 hypothetical protein -
LGM09_RS03620 (731667) 731667..731883 + 217 Protein_712 transposase -
LGM09_RS03630 (732561) 732561..733274 - 714 WP_002916694.1 DUF554 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 731667..731819 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17585.56 Da        Isoelectric Point: 8.5144

>T219634 WP_004889762.1 NZ_CP084866:729788-730273 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDSMMLMVTLRDLVN
A

Download         Length: 486 bp

>T219634 NZ_CP116564:c2654838-2654695 [Enterococcus faecalis]
ATGAGCGTACTTCCAAAATTTACAGAAAGGAGAGGCCTTTTGTCAGCATATGAAACAATTCAGACGATCCTAGGGTTTGG
TATGTTTACCATTGCTTTGATTGCGCTGATTGTTAAATTGCTTAAAAATGACAATAAAAAATAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT219634 WP_004150912.1 NZ_CP084866:729498-729791 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT219634 NZ_CP116564:2654577-2654762 [Enterococcus faecalis]
TGCTATAATAAAAACGAAAAGAGAGATATGCGTCAACATACCTCTCTGATGTAGAGCCGTTTAAGACGGTGACCATGTTA
ATTATTTAAAAATAACCGTGCTTGGTCAAAGTTGACGGTTATTTTTTATTGTCATTTTTAAGCAATTTAACAATCAGCGC
AATCAAAGCAATGGTAAACATACCAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A447W563

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References