Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 715996..716771 Replicon chromosome
Accession NZ_CP077779
Organism Klebsiella pneumoniae strain CDI231

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1W1JAQ8
Locus tag KUM55_RS03550 Protein ID WP_009308645.1
Coordinates 716286..716771 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag KUM55_RS03545 Protein ID WP_004150912.1
Coordinates 715996..716289 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KUM55_RS03525 (KUM55_03525) 711206..711808 - 603 WP_004174410.1 short chain dehydrogenase -
KUM55_RS03530 (KUM55_03530) 711906..712817 + 912 WP_004188550.1 LysR family transcriptional regulator -
KUM55_RS03535 (KUM55_03535) 712818..713966 - 1149 WP_040215095.1 PLP-dependent aspartate aminotransferase family protein -
KUM55_RS03540 (KUM55_03540) 713977..715353 - 1377 WP_004181304.1 cystathionine beta-synthase -
KUM55_RS03545 (KUM55_03545) 715996..716289 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
KUM55_RS03550 (KUM55_03550) 716286..716771 + 486 WP_009308645.1 GNAT family N-acetyltransferase Toxin
KUM55_RS03555 (KUM55_03555) 717475..718069 + 595 Protein_697 hypothetical protein -
KUM55_RS03560 (KUM55_03560) 718166..718382 + 217 Protein_698 transposase -
KUM55_RS03570 (KUM55_03570) 719060..719773 - 714 WP_025367888.1 DUF554 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 718166..718318 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17595.60 Da        Isoelectric Point: 8.5144

>T207800 WP_009308645.1 NZ_CP077779:716286-716771 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T207800 NZ_CP103515:1795026-1795277 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT207800 WP_004150912.1 NZ_CP077779:715996-716289 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT207800 NZ_CP103515:1795274-1795528 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1W1JAQ8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References