Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 2384838..2385613 Replicon chromosome
Accession NZ_CP051159
Organism Klebsiella pneumoniae strain 19PDR22

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag HF574_RS11985 Protein ID WP_004150910.1
Coordinates 2384838..2385323 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag HF574_RS11990 Protein ID WP_004150912.1
Coordinates 2385320..2385613 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HF574_RS11960 2380007..2381374 - 1368 WP_004150905.1 replicative DNA helicase -
HF574_RS11965 2381367..2381750 - 384 WP_004150906.1 hypothetical protein -
HF574_RS11970 2381750..2382622 - 873 WP_004188557.1 ParA family protein -
HF574_RS11975 2383228..2383444 - 217 Protein_2350 transposase -
HF574_RS11980 2383541..2384134 - 594 WP_004188553.1 hypothetical protein -
HF574_RS11985 2384838..2385323 - 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
HF574_RS11990 2385320..2385613 - 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
HF574_RS11995 2386258..2387634 + 1377 WP_004217775.1 cystathionine beta-synthase -
HF574_RS12000 2387645..2388793 + 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
HF574_RS12005 2388794..2389705 - 912 WP_004188550.1 LysR family transcriptional regulator -
HF574_RS12010 2389803..2390405 + 603 WP_062954968.1 short chain dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 2383292..2383444 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T153622 WP_004150910.1 NZ_CP051159:c2385323-2384838 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T153622 NZ_CP066993:c4667959-4667825 [Xanthomonas campestris pv. campestris]
ATGAAGCGTGCAATTGCATTGTTGGTGCTGTCGATGTTGTCGGTGGGAATGCTGGCGGGTTGCAACACCGTCGCAGGTGC
CGGCAAGGACGTGCAGGGCGCTGGCGAGAAGGTGGAAGACGCCGCGCGCAAGTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT153622 WP_004150912.1 NZ_CP051159:c2385613-2385320 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT153622 NZ_CP066993:c4668191-4668039 [Xanthomonas campestris pv. campestris]
ATGAAGCGACTGCTGACACTGATGGTGCTGGGCCTGTTCTCGGCCGGCATGATGACTGGCTGTAACACCATGGCTGGTGC
CGGAAAGGACATGCAGGGTGCGGGTGACAAGGTCGAGAAGACCGCCGACAAATGCAGCGACGGTAAGTGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References