Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 743371..744146 Replicon chromosome
Accession NZ_CP087606
Organism Klebsiella pneumoniae subsp. pneumoniae strain DD01304

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag LMH54_RS03720 Protein ID WP_004150910.1
Coordinates 743661..744146 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag LMH54_RS03715 Protein ID WP_004150912.1
Coordinates 743371..743664 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LMH54_RS03695 (LMH54_03695) 738579..739181 - 603 WP_062954968.1 short chain dehydrogenase -
LMH54_RS03700 (LMH54_03700) 739279..740190 + 912 WP_004188550.1 LysR family transcriptional regulator -
LMH54_RS03705 (LMH54_03705) 740191..741339 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
LMH54_RS03710 (LMH54_03710) 741350..742726 - 1377 WP_004217775.1 cystathionine beta-synthase -
LMH54_RS03715 (LMH54_03715) 743371..743664 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
LMH54_RS03720 (LMH54_03720) 743661..744146 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
LMH54_RS03725 (LMH54_03725) 744850..745443 + 594 WP_004188553.1 hypothetical protein -
LMH54_RS03730 (LMH54_03730) 745540..745756 + 217 Protein_733 transposase -
LMH54_RS03735 (LMH54_03735) 746362..747234 + 873 WP_004188557.1 ParA family protein -
LMH54_RS03740 (LMH54_03740) 747234..747617 + 384 WP_004150906.1 hypothetical protein -
LMH54_RS03745 (LMH54_03745) 747610..748977 + 1368 WP_004150905.1 SPI-7-type island replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 745540..745692 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T225629 WP_004150910.1 NZ_CP087606:743661-744146 [Klebsiella pneumoniae subsp. pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T225629 NZ_CP121223:3822478-3822633 [Shigella flexneri 2a]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT225629 WP_004150912.1 NZ_CP087606:743371-743664 [Klebsiella pneumoniae subsp. pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT225629 NZ_CP121223:c3822466-3822408 [Shigella flexneri 2a]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATTTGTGAGACATAGATTGGGCCTCA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References