Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 741190..741965 Replicon chromosome
Accession NZ_CP073002
Organism Klebsiella pneumoniae strain FO15

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag KBT91_RS03705 Protein ID WP_004150910.1
Coordinates 741480..741965 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag KBT91_RS03700 Protein ID WP_004150912.1
Coordinates 741190..741483 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KBT91_RS03680 736398..737000 - 603 WP_062954968.1 short chain dehydrogenase -
KBT91_RS03685 737098..738009 + 912 WP_004188550.1 LysR family transcriptional regulator -
KBT91_RS03690 738010..739158 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
KBT91_RS03695 739169..740545 - 1377 WP_004217775.1 cystathionine beta-synthase -
KBT91_RS03700 741190..741483 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
KBT91_RS03705 741480..741965 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
KBT91_RS03710 742669..743262 + 594 WP_004188553.1 hypothetical protein -
KBT91_RS03715 743359..743575 + 217 Protein_730 transposase -
KBT91_RS03720 744181..745053 + 873 WP_004188557.1 ParA family protein -
KBT91_RS03725 745053..745436 + 384 WP_004150906.1 hypothetical protein -
KBT91_RS03730 745429..746796 + 1368 WP_004150905.1 SPI-7-type island replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 743359..743511 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T199461 WP_004150910.1 NZ_CP073002:741480-741965 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T199461 NZ_CP097360:188058-188165 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT199461 WP_004150912.1 NZ_CP073002:741190-741483 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT199461 NZ_CP097360:c188009-187944 [Escherichia coli]
GTCTAGATGCAAGAATGGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References