50066

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 4530147..4530922 Replicon chromosome
Accession NZ_CP009863
Organism Klebsiella pneumoniae subsp. pneumoniae strain KPNIH29

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A332L402
Locus tag KPNIH29_RS22515 Protein ID WP_021314147.1
Coordinates 4530147..4530632 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag KPNIH29_RS22520 Protein ID WP_004150912.1
Coordinates 4530629..4530922 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KPNIH29_RS22495 4527145..4527858 + 714 WP_002916694.1 DUF554 domain-containing protein -
KPNIH29_RS22505 4528537..4528753 - 217 Protein_4299 transposase -
KPNIH29_RS22510 4528850..4529443 - 594 WP_038435706.1 hypothetical protein -
KPNIH29_RS22515 4530147..4530632 - 486 WP_021314147.1 GNAT family N-acetyltransferase Toxin
KPNIH29_RS22520 4530629..4530922 - 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
KPNIH29_RS22525 4531567..4532943 + 1377 WP_072269297.1 cystathionine beta-synthase -
KPNIH29_RS22530 4532954..4534102 + 1149 WP_038435707.1 PLP-dependent aspartate aminotransferase family protein -
KPNIH29_RS22535 4534103..4535014 - 912 WP_004181308.1 LysR family transcriptional regulator -
KPNIH29_RS22540 4535112..4535714 + 603 WP_038435708.1 short chain dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 4528601..4528753 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17621.68 Da        Isoelectric Point: 8.5144

>T50066 WP_021314147.1 NZ_CP009863:c4530632-4530147 [Klebsiella pneumoniae subsp. pneumoniae]
MILAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T50066 NZ_CP009863:c4530632-4530147 [Klebsiella pneumoniae subsp. pneumoniae]
ATGATCTTGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGACCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCGCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT50066 WP_004150912.1 NZ_CP009863:c4530922-4530629 [Klebsiella pneumoniae subsp. pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT50066 NZ_CP009863:c4530922-4530629 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A332L402

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References