Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 745023..745798 Replicon chromosome
Accession NZ_CP064241
Organism Klebsiella pneumoniae strain JX-CR-hvKP-3

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag IS502_RS03720 Protein ID WP_004150910.1
Coordinates 745313..745798 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag IS502_RS03715 Protein ID WP_004150912.1
Coordinates 745023..745316 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IS502_RS03695 740231..740833 - 603 WP_062954968.1 short chain dehydrogenase -
IS502_RS03700 740931..741842 + 912 WP_004188550.1 LysR family transcriptional regulator -
IS502_RS03705 741843..742991 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
IS502_RS03710 743002..744378 - 1377 WP_004217775.1 cystathionine beta-synthase -
IS502_RS03715 745023..745316 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
IS502_RS03720 745313..745798 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
IS502_RS03725 746502..747095 + 594 WP_004188553.1 hypothetical protein -
IS502_RS03730 747192..747408 + 217 Protein_733 transposase -
IS502_RS03735 748014..748886 + 873 WP_004188557.1 ParA family protein -
IS502_RS03740 748886..749269 + 384 WP_004150906.1 hypothetical protein -
IS502_RS03745 749262..750629 + 1368 WP_004150905.1 replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 747192..747344 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T180669 WP_004150910.1 NZ_CP064241:745313-745798 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T180669 NZ_CP085633:430165-431499 [Pectobacterium brasiliense]
ATGCCTCGAACACAACAGCGCTTAGCGATATGGATGAACGGGATCCAAGTTGGATTTTGGGAAAAGGCCAGAGGCGAAGA
TAGGTTGCAATACCTTCCCGAATGGATTGCTGATGAACAGGGAAGACCTTTATCGCTTTCTTTGCTTTTCACCCCCGGTA
ATCAGATTTGGCGCGGCAATGTGGTCCGCGACTATTTTGATAATTTATTGCCTGACAGCGAAGGTATACGCAGGCGTTTA
GCAACGCGTTACCATGCTGATAGCCTTGAGCCTTTTGATCTGTTGGCTGAGCTGGGGAGAGACTGTGTTGGTGCGATACA
GCTGCTGAATGGTAATGAGGAGCCTACAGATCTCTTTTCCGTAAAGTATCGCCCGCTTTGTGACGCTGAGATCGCCGCTA
TATTGCGTAATACAACAGCGGCATTGTTGCCAGGTCGGCAGGATGATACTGACGATTTACGTTTATCGATTGCCGGTGCG
CAGGAAAAAACGGCTTTACTCTGGCATGAAGGACAATGGTGCTTGCCAGAAGGAAGTACCCCAACGACGCATATTTTCAA
GTTACCACTCGGGCTAGTGGGTAATATGCAAGCGGATATGAGTACGTCGGTTGAAAATGAATGGCTATGTTCTTTGATCC
TTAAGGAATATGGGATTCCTGTCGCAAAAACGCAAATTGCACAGTTTGAAGAGCAGAAGGCACTGGTTGTTGAGCGTTTT
GACAGAAGATGGTCAAGCGATCGGCAATGGATCATTCGCTTGCCACAAGAGGATATGTGTCAGGCTCTGGGTGTTTCCCC
GCTACGAAAATACCAGTCTGATGGTGGGCCGGGGATTTCCGATATTATGACCATACTGAGTCATTCAGAGCAGGCTGAGC
AGGACAAAGCGCAGTTTTTCAGGGCGCAAATTATTTTCTGGCTGATAGCGGCTACTGATGGGCACGCCAAAAATTTCAGT
ATCGCTATTGAGCCACAAGGTCGCTATCACCTTACGCCACTTTATGATGTTTTATCGGCATGGCCTGTCATTGGTCCGCG
TAATAACCAGATTTCCTGGCAGAAGTGCAAGCTGGCAATGGCTGTTCGGGGTAGCAGTAATTATTACCATCTGTACAGAA
TTAAACGGCGGCATTGGGTCAATCATGGTGAATTAACTGGTCTGGGCAGACGACAGGTTGAAGCCATGATGGATGATATT
ATATCCAGGACACCTGAGGTTATTGAACGTGTATCTTCGTTGCTGCCTGAGTCATTCCCACCAGAGCTTGCGGAATGTGT
TTTTGACGGTATGCGACAACAGTGTGGTCGGTTACAAGAATTGCAGCGTAAATAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT180669 WP_004150912.1 NZ_CP064241:745023-745316 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT180669 NZ_CP085633:429855-430160 [Pectobacterium brasiliense]
ATGAATACAGTCTATCCGTTGAAAACCTTAAATCAGTTGCGCCCATTGCTGATTGGCTTTCGCAAAGCTAAGGGGCTGAC
GCAAAAAGACGTGTCTGAAAGGTTAGGTGTAACACAGCAAACCTATGCCCGCTTGGAAGCAAACCCCGGCAGTGCCAGTA
TTGAGCGCTTGTTTAAAGTGCTTACTGTTCTGGGGGTTGAGGTGGTGCTGTCTTCTGGACCAAACGTACATTTCTCTATG
TCATCCTCAACAATGGAAGAGCCAGAAAAGCCAACAGACTCACCCGCCAGACGGGAAAAATGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References