Detailed information of TA system TA153282

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 733042..733817
• Replicon: chromosome
• Accession: NZ_CP050840
• Organism: Klebsiella pneumoniae strain Bckp101

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: HC692_RS03670
• Protein ID: WP_108978882.1
• Coordinates: 733332..733817 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: W8UEW1
• Locus tag: HC692_RS03665
• Protein ID: WP_004150912.1
• Coordinates: 733042..733335 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HC692_RS03645	728250..728852	-	603	WP_002916735.1	short chain dehydrogenase	-
HC692_RS03650	728950..729861	+	912	WP_004188550.1	LysR family transcriptional regulator	-
HC692_RS03655	729862..731010	-	1149	WP_032417597.1	PLP-dependent aspartate aminotransferase family protein	-
HC692_RS03660	731021..732397	-	1377	WP_004217775.1	cystathionine beta-synthase	-
HC692_RS03665	733042..733335	+	294	WP_004150912.1	DUF1778 domain-containing protein	Antitoxin
HC692_RS03670	733332..733817	+	486	WP_108978882.1	GNAT family N-acetyltransferase	Toxin
HC692_RS03675	734509..735102	+	594	WP_108978883.1	hypothetical protein	-
HC692_RS03680	735199..735415	+	217	Protein_713	transposase	-
HC692_RS03690	735930..736643	-	714	WP_002916694.1	DUF554 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_016845 Klebsiella pneumoniae subsp. pneumoniae HS11286 IS/Tn TA026030 4559372..4559524 0	flank	IS/Tn	-	-	735199..735351	152

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (41-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (3-95)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17611.56 Da
• Isoelectric Point: 8.1550

>T153282 WP_108978882.1 NZ_CP050840:733332-733817 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQDTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Nucleotide

• Length: 486 bp

>T153282 NZ_CP066836:3455131-3455316 [Escherichia coli]
GTGAAAAGTGCAGACGTGATCGCCATCCTGAAGCAGCATGGCTGGGAACATATTCGAACCCGTGGCAGTCATCATCAGTT
CCGCCACCCTGTTCATCCGGGTCTGGTGACGGTTCCGCACCCTAAAAAAGATATTAAGCCCGGTACGCTGGCGCAAATTG
GGCGTCAGGCTGGTTTAACATTTTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10901.46 Da
• Isoelectric Point: 6.3783

>AT153282 WP_004150912.1 NZ_CP050840:733042-733335 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Nucleotide

• Length: 294 bp

>AT153282 NZ_CP066836:3455333-3455743 [Escherichia coli]
ATGTTTTATCCCGCCTATATCCACAGTGATCCTGACGGCAGCGCCAGCGGTTTTTTCCCTGATGTTCCAGGCTGTTATTT
TGCTGGTGATACGCTGGACAACGCTTTCCAGGATGCGCGAAGTGCACTGGTCGCGCACTTCGAAACCTTATGCGAGATGA
ATGAAGAGTTGCCGCTTCCAGGCTCTGTGGAAACACATCTGGTCCAGCGTGCGCAGGACTTTATTGGCGGGCAATGGTTG
CTGGTGGATATCAATATGAATCAGTTCGAAGGTCGGGCTGAACGTATCAATATTACGATGCCGAAAAGGTTACTGAACAA
GATTGATACCTACGTGCGCAATAACCCAGACTACGCCAACAGAAGTGCGTTTCTGGCAGAAGCTGCCCGGCGTGTATTGC
CGGGCGTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GVL4