Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 3202469..3203244 Replicon chromosome
Accession NZ_CP063881
Organism Klebsiella pneumoniae strain S90-2

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag IOD49_RS15675 Protein ID WP_004150910.1
Coordinates 3202759..3203244 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag IOD49_RS15670 Protein ID WP_004150912.1
Coordinates 3202469..3202762 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IOD49_RS15650 3197677..3198279 - 603 WP_002916735.1 short chain dehydrogenase -
IOD49_RS15655 3198377..3199288 + 912 WP_004188550.1 LysR family transcriptional regulator -
IOD49_RS15660 3199289..3200437 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
IOD49_RS15665 3200448..3201824 - 1377 WP_004217775.1 cystathionine beta-synthase -
IOD49_RS15670 3202469..3202762 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
IOD49_RS15675 3202759..3203244 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
IOD49_RS15680 3203948..3204541 + 594 WP_004188553.1 hypothetical protein -
IOD49_RS15685 3204638..3204854 + 217 Protein_3056 transposase -
IOD49_RS15690 3205460..3206332 + 873 WP_004188557.1 ParA family protein -
IOD49_RS15695 3206332..3206715 + 384 WP_004150906.1 hypothetical protein -
IOD49_RS15700 3206708..3208075 + 1368 WP_004150905.1 replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 3204638..3204790 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T179169 WP_004150910.1 NZ_CP063881:3202759-3203244 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T179169 NZ_CP084639:c974736-974533 [Yersinia ruckeri]
ATGACCAAAACTGACTACCTAATACGTTTAAGAAAATGCACAACAATTGACACACTAGAGCGTGTTATTGAAAAAAATAA
GTATGAGCTATCTGATGATGAACTAGAATTATTCTATTCTGCTGCGGATCATCGTCTAGCTGAACTTACCATGAATAAGT
TATACGATAAAATTCCTGCAACTGTCTGGCAACATGTGAAGTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT179169 WP_004150912.1 NZ_CP063881:3202469-3202762 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT179169 NZ_CP084639:c975156-974788 [Yersinia ruckeri]
ATGGATGAGTACTCACCTAAGCGACATGATATTGCTCAGTTGAAATTCTTATGTGAGAATTTGTATGATGAAGGTATCGC
CACATTGGGCGATAGCCACCACGGCTGGGTTAATGATCCAACCTCCGCGGTAAATCTCCAACTGAATGATTTAATCGAAC
ACATAGCTTCATTTGTGATGAGTTTTAAAATAAAATACGCAGACGAAATCGATCTCTGCGAGTTGGTGGAGGAATATCTA
GACGATACATATACCTTGTTCAGCAGTTACGGAATCAATGATCCTGATTTACGCCGCTGGCAGAAAACAAAAGACCGATT
ATTCAGACTATTTTCAGGGGAATACCTATGTGCCCTGATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References