Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 736488..737263 Replicon chromosome
Accession NZ_CP084986
Organism Klebsiella pneumoniae strain KP18-2110-2

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag LH404_RS03680 Protein ID WP_004150910.1
Coordinates 736778..737263 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag LH404_RS03675 Protein ID WP_004150912.1
Coordinates 736488..736781 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LH404_RS03655 731696..732298 - 603 WP_002916735.1 short chain dehydrogenase -
LH404_RS03660 732396..733307 + 912 WP_004188550.1 LysR family transcriptional regulator -
LH404_RS03665 733308..734456 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
LH404_RS03670 734467..735843 - 1377 WP_004217775.1 cystathionine beta-synthase -
LH404_RS03675 736488..736781 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
LH404_RS03680 736778..737263 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
LH404_RS03685 737967..738560 + 594 WP_004188553.1 hypothetical protein -
LH404_RS03690 738657..738873 + 217 Protein_725 transposase -
LH404_RS03695 739479..740351 + 873 WP_004188557.1 ParA family protein -
LH404_RS03700 740351..740734 + 384 WP_004150906.1 hypothetical protein -
LH404_RS03705 740727..742094 + 1368 WP_004150905.1 SPI-7-type island replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 738657..738809 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T220199 WP_004150910.1 NZ_CP084986:736778-737263 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T220199 NZ_CP116979:c23884-23603 [Escherichia coli]
ATGACCTATGAACTGGCTTTTGACCGTAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAACTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATACCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCCGTTGGAAGA
AGGGAAAGCAGCGAAGTCTATCAGATCGCAGATTTGCGCTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT220199 WP_004150912.1 NZ_CP084986:736488-736781 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT220199 NZ_CP116979:c24125-23874 [Escherichia coli]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References