Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 741309..742084 Replicon chromosome
Accession NZ_CP048933
Organism Klebsiella pneumoniae strain KP18-2079

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag G5B45_RS03760 Protein ID WP_004150910.1
Coordinates 741599..742084 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag G5B45_RS03755 Protein ID WP_004150912.1
Coordinates 741309..741602 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G5B45_RS03735 736517..737119 - 603 WP_062954968.1 short chain dehydrogenase -
G5B45_RS03740 737217..738128 + 912 WP_004188550.1 LysR family transcriptional regulator -
G5B45_RS03745 738129..739277 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
G5B45_RS03750 739288..740664 - 1377 WP_004217775.1 cystathionine beta-synthase -
G5B45_RS03755 741309..741602 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
G5B45_RS03760 741599..742084 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
G5B45_RS03765 742788..743381 + 594 WP_004188553.1 hypothetical protein -
G5B45_RS03770 743478..743694 + 217 Protein_729 transposase -
G5B45_RS03775 744300..745172 + 873 WP_004188557.1 ParA family protein -
G5B45_RS03780 745172..745555 + 384 WP_004150906.1 hypothetical protein -
G5B45_RS03785 745548..746915 + 1368 WP_004150905.1 replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 743478..743630 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T150231 WP_004150910.1 NZ_CP048933:741599-742084 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T150231 NZ_CP064405:2887863-2888303 [Mycobacterium tuberculosis variant bovis BCG]
ATGCTGCTCTGTGACACCAACATCTGGCTGGCGTTGGCGCTTTCCGGACACGTGCACCACAGGGCCTCGCGCGCATGGCT
AGACACCATCAACGCGCCCGGAGTCATCCACTTTTGCCGCGCAACCCAACAGTCGCTCCTTCGGCTGTTGACGAATCGGA
CGGTGCTGGGCGCGTATGGCAGCCCACCACTGACCAACCGCGAAGCGTGGGCGGCCTATGCCGCGTTCCTGGATGACGAC
CGCATCGTGCTGGCCGGCGCCGAACCTGATGGTTTGGAGGCCCAGTGGAGAGCCTTCGCCGTTCGCCAGTCGCCGGCGCC
CAAGGTTTGGATGGATGCCTACCTAGCTGCTTTCGCACTTACCGGTGGATTCGAGTTGGTGACGACTGACACCGCCTTCA
CCCAGTACGGCGGAATCGAGCTGCGGCTCCTGGCCAAGTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT150231 WP_004150912.1 NZ_CP048933:741309-741602 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT150231 NZ_CP064405:2887589-2887876 [Mycobacterium tuberculosis variant bovis BCG]
ATGAAGACCACGCTCGACCTGCCTGATGAACTGATGCGCGCTATCAAGGTCCGCGCGGCGCAGCAGGGCCGCAAGATGAA
AGATGTCGTGACCGAACTGCTCAGATCCGGTCTGTCCCAGACGCACAGCGGGGCTCCAATCCCAACGCCGCGGCGCGTGC
AGCTTCCCCTGGTGCATTGCGGTGGCGCGGCTACCCGCGAACAAGAAATGACGCCGGAGCGTGTTGCCGCGGCCTTGCTC
GACCAGGAGGCCCAGTGGTGGTCCGGACACGACGATGCTGCTCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References