Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 717098..717873 Replicon chromosome
Accession NZ_CP055057
Organism Klebsiella pneumoniae strain SWHE4

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A332L402
Locus tag HUZ67_RS03565 Protein ID WP_021314147.1
Coordinates 717388..717873 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag HUZ67_RS03560 Protein ID WP_004150912.1
Coordinates 717098..717391 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ67_RS03540 (HUZ67_03535) 712306..712908 - 603 WP_004174410.1 short chain dehydrogenase -
HUZ67_RS03545 (HUZ67_03540) 713006..713917 + 912 WP_021314148.1 LysR family transcriptional regulator -
HUZ67_RS03550 (HUZ67_03545) 713918..715066 - 1149 WP_239613194.1 PLP-dependent aspartate aminotransferase family protein -
HUZ67_RS03555 (HUZ67_03550) 715077..716453 - 1377 WP_004174417.1 pyridoxal-phosphate dependent enzyme -
HUZ67_RS03560 (HUZ67_03555) 717098..717391 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
HUZ67_RS03565 (HUZ67_03560) 717388..717873 + 486 WP_021314147.1 GNAT family N-acetyltransferase Toxin
HUZ67_RS03570 (HUZ67_03565) 718577..719170 + 594 WP_004188553.1 hypothetical protein -
HUZ67_RS03575 (HUZ67_03570) 719267..719483 + 217 Protein_702 transposase -
HUZ67_RS03585 (HUZ67_03580) 719998..720711 - 714 WP_002916694.1 DUF554 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 719267..719419 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17621.68 Da        Isoelectric Point: 8.5144

>T161278 WP_021314147.1 NZ_CP055057:717388-717873 [Klebsiella pneumoniae]
MILAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T161278 NZ_CP071127:2808307-2808732 [Mycobacterium tuberculosis]
GTGGCGCTGCTCGACGTCAACGCATTGGTCGCGCTGGCGTGGGACTCACACATCCACCACGCCCGGATCCGCGAGTGGTT
TACCGCCAACGCCACGCTCGGCTGGGCGACTTGCCCGCTCACCGAAGCCGGCTTCGTGCGGGAGTCGACGAACCCAAAAG
TACTTCCCAGCGCGATCGGGATCGCAGACGCTCGACGGGTCCTCGTGGCACTACGCGCCGTGGGAGGCCACCGCTTCCTG
GCTGACGACGTATCGCTCGTCGATGACGATGTTCCGTTGATCGTCGGTTATCGCCAGGTGACCGACGCCCATCTGCTGAC
ACTCGCCCGCCGGCGCGGCGTCCGCCTGGTCACCTTCGACGCCGGTGTCTTCACCCTCGCCCAACAACGCCCCAAGACGC
CAGTGGAGCTGCTGACCATCCTCTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT161278 WP_004150912.1 NZ_CP055057:717098-717391 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT161278 NZ_CP071127:2808080-2808301 [Mycobacterium tuberculosis]
GTGCGAACGACACTTGACCTTGACGACGATGTGATCGCCGCGGCACGTGAACTTGCCTCCAGCCAGCGCCGCTCGCTCGG
CTCGGTGATTTCCGAACTCGCACGCCGTGGTCTCATGCCCGGACGCGTCGAGGCTGACGACGGGCTGCCGGTGATCCGCG
TTCCAGCCGGGACCCCGCCGATCACACCGGAGATGGTCCGTCGCGCGCTCGATGAGGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A332L402

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References