Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 738658..739433 Replicon chromosome
Accession NZ_CP093495
Organism Klebsiella pneumoniae strain VA32

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag MO414_RS03695 Protein ID WP_004150910.1
Coordinates 738948..739433 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag MO414_RS03690 Protein ID WP_004150912.1
Coordinates 738658..738951 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MO414_RS03670 (MO414_03670) 733866..734468 - 603 WP_002916735.1 short chain dehydrogenase -
MO414_RS03675 (MO414_03675) 734566..735477 + 912 WP_004188550.1 LysR family transcriptional regulator -
MO414_RS03680 (MO414_03680) 735478..736626 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
MO414_RS03685 (MO414_03685) 736637..738013 - 1377 WP_004217775.1 cystathionine beta-synthase -
MO414_RS03690 (MO414_03690) 738658..738951 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
MO414_RS03695 (MO414_03695) 738948..739433 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
MO414_RS03700 (MO414_03700) 740137..740730 + 594 WP_004188553.1 hypothetical protein -
MO414_RS03705 (MO414_03705) 740827..741043 + 217 Protein_728 transposase -
MO414_RS03710 (MO414_03710) 741649..742521 + 873 WP_004188557.1 ParA family protein -
MO414_RS03715 (MO414_03715) 742521..742904 + 384 WP_004150906.1 hypothetical protein -
MO414_RS03720 (MO414_03720) 742897..744264 + 1368 WP_004150905.1 SPI-7-type island replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 740827..740979 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T238776 WP_004150910.1 NZ_CP093495:738948-739433 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T238776 NZ_LR890471:c4824807-4824523 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTATGCAGAATCTGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT238776 WP_004150912.1 NZ_CP093495:738658-738951 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT238776 NZ_LR890471:c4825039-4824797 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References