Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 4662078..4662853 Replicon chromosome
Accession NZ_CP058752
Organism Klebsiella pneumoniae strain STLIN_19

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1W1JAQ8
Locus tag HZT05_RS22900 Protein ID WP_009308645.1
Coordinates 4662368..4662853 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag HZT05_RS22895 Protein ID WP_004150912.1
Coordinates 4662078..4662371 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZT05_RS22875 (4657286) 4657286..4657888 - 603 WP_002916735.1 short chain dehydrogenase -
HZT05_RS22880 (4657986) 4657986..4658897 + 912 WP_021314148.1 LysR family transcriptional regulator -
HZT05_RS22885 (4658898) 4658898..4660046 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
HZT05_RS22890 (4660057) 4660057..4661433 - 1377 WP_004217775.1 cystathionine beta-synthase -
HZT05_RS22895 (4662078) 4662078..4662371 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
HZT05_RS22900 (4662368) 4662368..4662853 + 486 WP_009308645.1 GNAT family N-acetyltransferase Toxin
HZT05_RS22905 (4663557) 4663557..4664150 + 594 WP_004188553.1 hypothetical protein -
HZT05_RS22910 (4664247) 4664247..4664463 + 217 Protein_4479 transposase -
HZT05_RS22915 (4665070) 4665070..4665942 + 873 WP_004188557.1 ParA family protein -
HZT05_RS22920 (4665942) 4665942..4666325 + 384 WP_004150906.1 hypothetical protein -
HZT05_RS22925 (4666318) 4666318..4667685 + 1368 WP_004150905.1 SPI-7-type island replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 4664247..4664399 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17595.60 Da        Isoelectric Point: 8.5144

>T167382 WP_009308645.1 NZ_CP058752:4662368-4662853 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T167382 NZ_CP075120:3595998-3596255 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGAATGCCTCCGGCGTCAGCGTCCGCCACATCAACAGCAAAACCCACATGACCACCTATTACTCACAAATCCCCAGCCT
GCATCTTAAGGGCGACTGGCTGGAAGAAGCGGGATTTAAGACCGGGCGCGGCGTCACCGTGAAGATTTCACAGGGGTGTA
TTGTGCTGATGGCGGACAGTAACGAGGAGCAGAAGCTGCGCGAGCAGCTTTATCAGGCTAAACAGGTGGTTAAGGGAATT
AAGGACGTGCTGGTTTAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT167382 WP_004150912.1 NZ_CP058752:4662078-4662371 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT167382 NZ_CP075120:c3596173-3595841 [Salmonella enterica subsp. enterica serovar Enteritidis]
TCCGCCATCAGCACAATACACCCCTGTGAAATCTTCACGGTGACGCCGCGCCCGGTCTTAAATCCCGCTTCTTCCAGCCA
GTCGCCCTTAAGATGCAGGCTGGGGATTTGTGAGTAATAGGTGGTCATGTGGGTTTTGCTGTTGATGTGGCGGACGCTGA
CGCCGGAGGCATTCATATTTGCTGACTAAATAAATTCTTAATTATCCGCCGGATGCTGGCTGATTGTGGAGCTCAGGGTG
AGCGAGTATGGGCGCGACATCCTGGCACTGAGCCCCCCTCTCCCCGGCCAGCTCCGGGCGGTGATGAGCAACCGGCTGCC
GGGGCCGTATTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1W1JAQ8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References