Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 4550326..4551101 Replicon chromosome
Accession NZ_CP110936
Organism Klebsiella pneumoniae subsp. pneumoniae strain LC-1736/18

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag NPY06_RS22505 Protein ID WP_004150910.1
Coordinates 4550326..4550811 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag NPY06_RS22510 Protein ID WP_004150912.1
Coordinates 4550808..4551101 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NPY06_RS22480 (NPY06_22480) 4545496..4546863 - 1368 WP_004150905.1 SPI-7-type island replicative DNA helicase -
NPY06_RS22485 (NPY06_22485) 4546856..4547239 - 384 WP_004150906.1 hypothetical protein -
NPY06_RS22490 (NPY06_22490) 4547239..4548111 - 873 WP_004188557.1 ParA family protein -
NPY06_RS22495 (NPY06_22495) 4548717..4548933 - 217 Protein_4396 transposase -
NPY06_RS22500 (NPY06_22500) 4549030..4549622 - 593 Protein_4397 hypothetical protein -
NPY06_RS22505 (NPY06_22505) 4550326..4550811 - 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
NPY06_RS22510 (NPY06_22510) 4550808..4551101 - 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
NPY06_RS22515 (NPY06_22515) 4551746..4553122 + 1377 WP_004217775.1 cystathionine beta-synthase -
NPY06_RS22520 (NPY06_22520) 4553133..4554281 + 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
NPY06_RS22525 (NPY06_22525) 4554282..4555193 - 912 WP_004188550.1 LysR family transcriptional regulator -
NPY06_RS22530 (NPY06_22530) 4555291..4555893 + 603 WP_002916735.1 short chain dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Integrative and Conjugative Element - - 4493309..4551352 58043
flank IS/Tn - - 4548781..4548933 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T264536 WP_004150910.1 NZ_CP110936:c4550811-4550326 [Klebsiella pneumoniae subsp. pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT264536 WP_004150912.1 NZ_CP110936:c4551101-4550808 [Klebsiella pneumoniae subsp. pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References