Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 723752..724527 Replicon chromosome
Accession NZ_CP081891
Organism Klebsiella pneumoniae strain CT-04

Toxin (Protein)


Gene name TacT2 Uniprot ID B6ZBI6
Locus tag KZ663_RS03585 Protein ID WP_004889762.1
Coordinates 724042..724527 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag KZ663_RS03580 Protein ID WP_004150912.1
Coordinates 723752..724045 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZ663_RS03560 (KZ663_03555) 718961..719563 - 603 WP_004174410.1 short chain dehydrogenase -
KZ663_RS03565 (KZ663_03560) 719661..720572 + 912 WP_017879796.1 LysR family transcriptional regulator -
KZ663_RS03570 (KZ663_03565) 720573..721721 - 1149 WP_004889759.1 PLP-dependent aspartate aminotransferase family protein -
KZ663_RS03575 (KZ663_03570) 721732..723108 - 1377 WP_017879795.1 pyridoxal-phosphate dependent enzyme -
KZ663_RS03580 (KZ663_03575) 723752..724045 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
KZ663_RS03585 (KZ663_03580) 724042..724527 + 486 WP_004889762.1 GNAT family N-acetyltransferase Toxin
KZ663_RS03590 (KZ663_03585) 725231..725823 + 593 Protein_706 hypothetical protein -
KZ663_RS03595 (KZ663_03590) 725920..726136 + 217 Protein_707 transposase -
KZ663_RS03605 (KZ663_03600) 726651..727364 - 714 WP_002916694.1 DUF554 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 725920..726072 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17585.56 Da        Isoelectric Point: 8.5144

>T213678 WP_004889762.1 NZ_CP081891:724042-724527 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDSMMLMVTLRDLVN
A

Download         Length: 486 bp

>T213678 NZ_CP109775:c1753187-1753080 [Shigella sonnei]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT213678 WP_004150912.1 NZ_CP081891:723752-724045 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT213678 NZ_CP109775:1753232-1753298 [Shigella sonnei]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A447W563

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References