Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 4941474..4942249 Replicon chromosome
Accession NZ_CP090203
Organism Klebsiella pneumoniae strain JNKPN26

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag LXM99_RS24270 Protein ID WP_004150910.1
Coordinates 4941764..4942249 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag LXM99_RS24265 Protein ID WP_004150912.1
Coordinates 4941474..4941767 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LXM99_RS24245 (LXM99_24245) 4936682..4937284 - 603 WP_062954968.1 short chain dehydrogenase -
LXM99_RS24250 (LXM99_24250) 4937382..4938293 + 912 WP_004188550.1 LysR family transcriptional regulator -
LXM99_RS24255 (LXM99_24255) 4938294..4939442 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
LXM99_RS24260 (LXM99_24260) 4939453..4940829 - 1377 WP_004217775.1 cystathionine beta-synthase -
LXM99_RS24265 (LXM99_24265) 4941474..4941767 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
LXM99_RS24270 (LXM99_24270) 4941764..4942249 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
LXM99_RS24275 (LXM99_24275) 4942953..4943546 + 594 WP_004188553.1 hypothetical protein -
LXM99_RS24280 (LXM99_24280) 4943643..4943859 + 217 Protein_4750 transposase -
LXM99_RS24285 (LXM99_24285) 4944465..4945337 + 873 WP_004188557.1 ParA family protein -
LXM99_RS24290 (LXM99_24290) 4945337..4945720 + 384 WP_004150906.1 hypothetical protein -
LXM99_RS24295 (LXM99_24295) 4945713..4947080 + 1368 WP_004150905.1 SPI-7-type island replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 4943643..4943795 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T230231 WP_004150910.1 NZ_CP090203:4941764-4942249 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T230231 NZ_CP124809:c322907-322590 [Citrobacter freundii]
ATGTTCACATTTATTGAGCTACAAGGATTTAGTAAACGCCGTCCGTTACTTTTGCCTGACGATGAGTTTCGCGCTTTTCA
AGAGGCGCTTATAGAGAACCCTGAGGCTGGAGATACTATCGCTGGTACAGGTGGATTCAGAAAAATTCGCTGGAGTCGCT
CTGGTATGGGCAAACGCAGCGGTATTAGGGTTATCTATTACAACGTGACGCGCAAAGGTCGAATCTATCTCGCTTTGCTC
TATCCTAAAAATGAACAGGATGATTTAACCGAAGAGCAAAAAAGAGTTTTAATGCACTTATCCAATATGCTGATTTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT230231 WP_004150912.1 NZ_CP090203:4941474-4941767 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT230231 NZ_CP124809:c322537-322241 [Citrobacter freundii]
ATGAAAGACGAATTATTTGCCGATTTGCTGGCCAGTGCAGAAGAAATGGTGCGTATAGAGAAAGGTGAAGAAACACCTAA
GCCTGAACATGTACATACCTTTAGCGAAATTGATGTTAAAGCCATCCGCGAAGCAACAGGGTTACGCCAACAGGATTTTG
CCATAGCTGTTGGCGTGAGTTATGACCTGGTTAAAAGCTGGGAAACGAAACGACGCCAGCCGACTGGTGCACCAAGAAAA
CTGCTTTTATTAATTCAAAAAAATCCATTTATAATTAATCAATTAAAAGCAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References