Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 741519..742294 Replicon chromosome
Accession NZ_CP078122
Organism Klebsiella pneumoniae strain KPC-2

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag KU889_RS03695 Protein ID WP_004150910.1
Coordinates 741809..742294 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag KU889_RS03690 Protein ID WP_004150912.1
Coordinates 741519..741812 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KU889_RS03670 736727..737329 - 603 WP_062954968.1 short chain dehydrogenase -
KU889_RS03675 737427..738338 + 912 WP_004188550.1 LysR family transcriptional regulator -
KU889_RS03680 738339..739487 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
KU889_RS03685 739498..740874 - 1377 WP_004217775.1 cystathionine beta-synthase -
KU889_RS03690 741519..741812 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
KU889_RS03695 741809..742294 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
KU889_RS03700 742998..743591 + 594 WP_004188553.1 hypothetical protein -
KU889_RS03705 743688..743904 + 217 Protein_729 transposase -
KU889_RS03710 744510..745382 + 873 WP_004188557.1 ParA family protein -
KU889_RS03715 745382..745765 + 384 WP_004150906.1 hypothetical protein -
KU889_RS03720 745758..747125 + 1368 WP_004150905.1 SPI-7-type island replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 743688..743840 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T209040 WP_004150910.1 NZ_CP078122:741809-742294 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T209040 NZ_CP103810:2008686-2009048 [Actinobacillus equuli subsp. haemolyticus]
GTGAAGTTAGTTTTTGTAGAATTACCACCTTTTGAGCGATATAGACAAAAGCACCTTAGTGATGAAGAATATAGACATTT
TCAGAATGAGCTATTAGAAAATCCTAATAAAGGAGATTTAATCCAAGGTACGAACGGCTTACGCAAAATTAGAATTGCAG
ATATGCAACGAAATAAAGGTAAGAGAGGTGGAGCAAGGGTAATTTATTATCATATTGTGAATTATTCAAAAATTTTGTTG
GTTACCGCATATGGTAAAGATGAACAATCAGATTTATCTAATACGGAACGCAATATGTTAGCGAATAAAGTGAAGCGTAT
TGTTGAATTAGAGATAAGGAGTTGTAATGGGAAAACTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT209040 WP_004150912.1 NZ_CP078122:741519-741812 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT209040 NZ_CP103810:2009032-2009340 [Actinobacillus equuli subsp. haemolyticus]
ATGGGAAAACTTTTTGATGATTTAATGGAAGGGCTTGATGCTTATGAAAAACATATCCAAGGTAAAATCACCCTGCGAAC
CACGGTATTAGAAAAGCCTGAACCAGTAGAACTTAGCCCGTCTGAAGTTAAATCGATTCGAGAAAAATTAAATCTGTCGC
AAGCGGTTTTTGCGCATAAATTGCATACTTCCGTGCGGACTTATCAAGGGTGGGAGCAAGGCAAAACTAAACCGAATCCA
CAAGCTACTTTGTTATTACGAATGGTGGACAAATCTCCGCAATTATTAGAACAAATGGCACAGCTCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References