Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 742551..743326 Replicon chromosome
Accession NZ_CP081820
Organism Klebsiella pneumoniae strain F726925

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag K5O13_RS03710 Protein ID WP_004150910.1
Coordinates 742841..743326 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag K5O13_RS03705 Protein ID WP_004150912.1
Coordinates 742551..742844 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K5O13_RS03685 (K5O13_03685) 737759..738361 - 603 WP_062954968.1 short chain dehydrogenase -
K5O13_RS03690 (K5O13_03690) 738459..739370 + 912 WP_004188550.1 LysR family transcriptional regulator -
K5O13_RS03695 (K5O13_03695) 739371..740519 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
K5O13_RS03700 (K5O13_03700) 740530..741906 - 1377 WP_004217775.1 cystathionine beta-synthase -
K5O13_RS03705 (K5O13_03705) 742551..742844 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
K5O13_RS03710 (K5O13_03710) 742841..743326 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
K5O13_RS03715 (K5O13_03715) 744030..744623 + 594 WP_004188553.1 hypothetical protein -
K5O13_RS03720 (K5O13_03720) 744720..744936 + 217 Protein_732 transposase -
K5O13_RS03725 (K5O13_03725) 745542..746414 + 873 WP_004188557.1 ParA family protein -
K5O13_RS03730 (K5O13_03730) 746414..746797 + 384 WP_004150906.1 hypothetical protein -
K5O13_RS03735 (K5O13_03735) 746790..748157 + 1368 WP_004150905.1 SPI-7-type island replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 744720..744872 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T213492 WP_004150910.1 NZ_CP081820:742841-743326 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T213492 NZ_CP109604:1912471-1912573 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT213492 WP_004150912.1 NZ_CP081820:742551-742844 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT213492 NZ_CP109604:c1912580-1912435 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References